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    Journal of Forensic Medicine    2023, 39 (3): 308-311.   DOI: 10.12116/j.issn.1004-5619.2021.510905
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    Journal of Forensic Medicine    2023, 39 (3): 229-230.   DOI: 10.12116/j.issn.1004-5619.2023.530501
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    Identification Strategy of Biological Half Sibling Relationship
    Zheng TAN, Guan-ju MA, Li-hong FU, Xiao-jing ZHANG, Qian WANG, Guang-ping FU, Qing-qing DU, Shu-jin LI
    Journal of Forensic Medicine    2023, 39 (3): 262-270.   DOI: 10.12116/j.issn.1004-5619.2023.530107
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    Objective To compare the application value of the likelihood ratio (LR) method and identity by state (IBS) method in the identification involving half sibling relationships, and to provide a reference for the setting of relevant standards for identification of half sibling relationship. Methods (1) Based on the same genetic marker combinations, the reliability of computer simulation method was verified by comparing the distributions of cumulated identity by state score (CIBS) and combined full sibling index in actual cases with the distributions in simulated cases. (2) In different numbers of three genetic marker combinations, the simulation of full sibling, half sibling and unrelated individual pairs, each 1 million pairs, was obtained; the CIBS, as well as the corresponding types of cumulative LR parameters, were calculated. (3) The application value of LR method was compared with that of IBS method, by comparing the best system efficiency provided by LR method and IBS method when genetic markers in different amounts and of different types and accuracy were applied to distinguish the above three relational individual pairs. (4) According to the existing simulation data, the minimum number of genetic markers required to distinguish half siblings from the other two relationships using different types of genetic markers was estimated by curve fitting. Results (1) After the rank sum test, under the premise that the real relationship and the genetic marker combination tested were the same, there was no significant difference between the simulation method and the results obtained in the actual case. (2) In most cases, under the same conditions, the system effectiveness obtained by LR method was greater than that by IBS method. (3) According to the existing data, the number of genetic markers required for full-half siblings and half sibling identification could be obtained by curve fitting when the system effectiveness reached 0.95 or 0.99. Conclusion When distinguishing half sibling from full sibling pairs or unrelated pairs, it is recommended to give preference to the LR method, and estimate the required number of markers according to the identification types and the population data, to ensure the identification effect.

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    Process of Forensic Medicine in DNA Identification of Aged Human Remains
    Lu CHEN, Zhe ZHOU, Sheng-qi WANG
    Journal of Forensic Medicine    2023, 39 (5): 478-486.   DOI: 10.12116/j.issn.1004-5619.2021.511209
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    Skeleton and teeth are important biological samples. Due to their special structure and strong ability to resist degradation, they are ideal biological materials to retain DNA under natural condition. In many cases, such as historical figure identification, aged skeleton and teeth are usually the only biological samples. However, their DNA is in a state of trace, damage and degradation to different degrees, which requires special experimental treatment to achieve identification. This paper reviews the sample selection, DNA extraction, DNA enrichment and analysis approaches based on relevant research reports in recent years, aiming to promote the further development and improvement of the aged skeleton and teeth identification system.

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    Retrospective Analysis of Death Cases of Oral Diphenidol Hydrochloride Poisoning
    Yu YANG, Fan-zhang LEI, Yu-you DONG, Jian-long MA, Qi-qiang SHI, Xue-song YE
    Journal of Forensic Medicine    2023, 39 (4): 393-398.   DOI: 10.12116/j.issn.1004-5619.2022.420401
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    Objective To explore the characteristics of postmortem examination, chemical examination and scene investigation of deaths caused by oral diphenidol hydrochloride poisoning, and so as to provide a reference for proper settlement and prevention of such deaths. Methods The data of 22 deaths caused by oral diphenidol hydrochloride poisoning in a city from January 2018 to August 2020 were collected, including case details, scene investigations, autopsies, chemical examinations and digital evidence. Thirty-one cases of deaths caused by oral diphenidol hydrochloride poisoning reported in previous literature were also collected. Results In the 53 oral diphenidol hydrochloride poisoning death cases, 50 cases were suicide, 2 cases were accidental, while 1 case was undetermined. Fifty-two cases were found in the medical records or crime scene investigation reports with doses ranging from 775 mg to 12 500 mg, and 23 deceased were detected with postmortem blood concentrations ranging from 2.71 mg/L to 83.1 mg/L. Clinical symptoms were recorded in 6 patients, including conscious disturbance and convulsion. Among the 45 cases which were performed with external examination, 23 cases autopsied. Conclusion Most of the deceased of oral diphenidol hydrochloride poisoning were suicide. No significant correlation was found between dose and blood concentration through the retrospective analysis of cases.

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    Methods and Research Hotspots of Forensic Kinship Testing
    Ran LI, Hong-yu SUN
    Journal of Forensic Medicine    2023, 39 (3): 231-239.   DOI: 10.12116/j.issn.1004-5619.2023.530208
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    Kinship testing is widely needed in forensic science practice. This paper reviews the definitions of common concepts, and summarizes the basic principles, advantages and disadvantages, and application scope of kinship analysis methods, including identity by state (IBS) method, likelihood ratio (LR) method, method of moment (MoM), and identity by descent (IBD) segment method. This paper also discusses the research hotspots of challenging kinship testing, complex kinship testing, forensic genetic genealogy analysis, and non-human biological samples.

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    Journal of Forensic Medicine    2023, 39 (4): 337-342.   DOI: 10.12116/j.issn.1004-5619.2023.230310
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    General Formulas for Calculating Commonly Used Kinship Index
    Chao XIAO, Dai-xin HUANG
    Journal of Forensic Medicine    2023, 39 (3): 276-282.   DOI: 10.12116/j.issn.1004-5619.2023.530104
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    Objective To derive general formulas for calculating commonly used kinship index (KI). Methods By introducing the Kronecker symbol, the formulas used to calculate the same KI under different genotype combinations were summarized into a unified expression. Results The general formulas were successfully derived for KI in various case situations, including the paternity index, full sibling index, half sibling index, avuncular index, grandpaternity index, first-cousin index, and second-cousin index between two individuals without or with the mother being involved; grandpaternity index between grandparents and a grandchild without or with the mother being involved; half sibling index between two children with two mothers being involved; full sibling index among three children; and half sibling index among three children with no, one, or two mothers being involved. Conclusion The general formulas given in this study simplify the calculation of KIs and facilitate fast and accurate calculation through programming.

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    Journal of Forensic Medicine    2023, 39 (5): 512-515.   DOI: 10.12116/j.issn.1004-5619.2022.420604
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    Journal of Forensic Medicine    2023, 39 (5): 528-530.   DOI: 10.12116/j.issn.1004-5619.2022.220214
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    Journal of Forensic Medicine    2023, 39 (6): 623-625.   DOI: 10.12116/j.issn.1004-5619.2022.421001
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    Research Progress and Application Prospect of Facial Micro-Expression Analysis in Forensic Psychiatry
    Wen LI, Hao-zhe LI, Chen CHEN, Wei-xiong CAI
    Journal of Forensic Medicine    2023, 39 (5): 493-500.   DOI: 10.12116/j.issn.1004-5619.2022.120104
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    Research on facial micro-expression analysis has been going on for decades. Micro-expression can reflect the true emotions of individuals, and it has important application value in assisting auxiliary diagnosis and disease monitoring of mental disorders. In recent years, the development of artificial intelligence and big data technology has made the automatic recognition of micro-expressions possible, which will make micro-expression analysis more convenient and more widely used. This paper reviews the development of facial micro-expression analysis and its application in forensic psychiatry, to look into further application prospects and development direction.

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    Research Progresses of Tri-Allelic Patterns in Autosomal STR in Forensic DNA Analysis
    Xiao-yan MA, Hong-yu SUN, Qing LI
    Journal of Forensic Medicine    2023, 39 (3): 240-246.   DOI: 10.12116/j.issn.1004-5619.2023.530210
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    Tri-allelic pattern in autosomal STR is a common abnormal typing phenomenon in forensic DNA analysis, which brings difficulties and uncertainties to the evaluation of the evidence weight in actual cases. This paper reviews the types, formation mechanism, occurrence frequency, genetic pattern and quantitative evaluation of evidence of the tri-allelic pattern in autosomal STR in forensic DNA analysis. This paper mainly explains the formation mechanism and genetic patterns based on different types of tri-allelic pattern. This paper also discusses the determination of tri-allelic pattern and the quantitative method of evidence evaluation in paternity testing and individual identification. This paper aims to provide references for scientific and standardized analysis of this abnormal typing phenomenon in forensic DNA analysis.

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    Journal of Forensic Medicine    2023, 39 (5): 509-511.   DOI: 10.12116/j.issn.1004-5619.2022.420903
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    Journal of Forensic Medicine    2023, 39 (4): 417-418.   DOI: 10.12116/j.issn.1004-5619.2022.420203
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    Journal of Forensic Medicine    2023, 39 (6): 626-627.   DOI: 10.12116/j.issn.1004-5619.2023.430602
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    Journal of Forensic Medicine    2023, 39 (5): 431-432.   DOI: 10.12116/j.issn.1004-5619.2023.431002
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    Legal Theories, Disability Models and Principles of Disability Assessment
    Xu WANG
    Journal of Forensic Medicine    2023, 39 (4): 329-336.   DOI: 10.12116/j.issn.1004-5619.2023.230307
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    In the personal injury compensation system, the protection and relief of the injured people’s rights to life, rights to health, and body rights are generally based on the results of disability assessment. Over the years, with the increased number of personal injury compensation cases, the practice of disability assessment have been greatly developed, and the development of disability assessment standards tends to be mature. However, the lack of basic theories for disability assessment has seriously affected the construction and unification of standards. Starting from the tort legal system of personal injury compensation, this article systematically analyzes the legal theories of disability assessment, and holds that the loss of labor ability is the legal basis for disability assessment in China, and the essence of disability assessment should be understood as the quantitative assessment of an individual’s permanent loss of labor ability. This article combines the international disability assessment models and the primary concepts of American Medical Association’s Guides to the Evaluation of Permanent Impairment to refine the basic concepts of disability assessment in China, such as impairment, disability, handicap, disabled people and self-care ability, etc. At the same time, it sorts out the critical issues of identification time, promotion principles and compound calculation of multiple injuries in disability assessment. It is expected to be beneficial to the theory and practice of disability assessment in personal injury compensation.

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    Journal of Forensic Medicine    2023, 39 (3): 312-314.   DOI: 10.12116/j.issn.1004-5619.2023.530103
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    Qualitative and Quantitative Analysis of Five Indoles or Indazole Amide Synthetic Cannabinoids in Suspected E-Cigarette Oil by GC-MS
    Cui-mei LIU, Wei JIA, Chun-hui SONG, Zhen-hua QIAN, Zhen-dong HUA, Yue-meng CHEN
    Journal of Forensic Medicine    2023, 39 (5): 457-464.   DOI: 10.12116/j.issn.1004-5619.2022.320101
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    Objective To establish the GC-MS qualitative and quantitative analysis methods for the synthetic cannabinoids, its main matrix and additives in suspicious electronic cigarette (e-cigarette) oil samples. Methods The e-cigarette oil samples were analyzed by GC-MS after diluted with methanol. Synthetic cannabinoids, its main matrix and additives in e-cigarette oil samples were qualitatively analyzed by the characteristic fragment ions and retention time. The synthetic cannabinoids were quantitatively analyzed by using the selective ion monitoring mode. Results The linear range of each compound in GC-MS quantitative method was 0.025-1 mg/mL, the matrix recovery rate was 94%-103%, the intra-day precision relative standard deviations (RSD) was less than 2.5%, and inter-day precision RSD was less than 4.0%. Five indoles or indazole amide synthetic cannabinoids were detected in 25 e-cigarette samples. The main matrixes of e-cigarette samples were propylene glycol and glycerol. Additives such as N,2,3-trimethyl-2-isopropyl butanamide (WS-23), glycerol triacetate and nicotine were detected in some samples. The content range of synthetic cannabinoids in 25 e-cigarette samples was 0.05%-2.74%. Conclusion The GC-MS method for synthesizing cannabinoid, matrix and additive in e-cigarette oil samples has good selectivity, high resolution, low detection limit, and can be used for simultaneous qualitative and quantitative analysis of multiple components; The explored fragment ion fragmentation mechanism of the electron bombardment ion source of indole or indoxamide compounds helps to identify such substances or other compounds with similar structures in cases.

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