法医学杂志 ›› 1985, Vol. 0 ›› Issue (1): 24-28.

• 论文 • 上一篇    下一篇

吗啡的免疫测定

胡炳蔚;   

  1. 西安医学院法医学教研室,
  • 发布日期:1985-02-25 出版日期:1985-02-28

Morphine Immunoassay

HU BINGWEI(DEPARTMENT OF FORENSIC MEDICINE XI'AN MEDICAL COLLEGE)   

  • Online:1985-02-25 Published:1985-02-28

摘要: <正> 利用抗原抗体反应的经典免疫化学程序已应用于毒物的检测。由于大部分毒物为低分子化合物,缺乏抗原性或仅为半抗原,不能诱发免疫反应。必须与大分子蛋白或合成的多肽等结合后方成为完全抗原,用它来免疫动物可产生对该毒物具有特异性的抗体。利用此特异性抗体可直接测定血、尿等生物检材中的毒物含量。采用此种测定法,测定尿

Abstract: Immunoassay for Poisons is based on Clssical immunochemical procedures and utilizes an antigen-antibody reaction as an analytic means. It can determine the poisons contained in the blood or urine Sample quantitatively and directly No isolation is necessary, Thus this procedure can be performed easily and rapidly, and the results obtained are sensitive. For instance, the sensitivity of radioimmunoassay is 10, 000~10, 000, 000 times as high as that of the triditional method, up to ng(10~(-12)). The main adventage of this method is that it can be used as a Screening test, especrally the negative exclusive tests. This paper presents morphine radioimmunoassay and enzyme inhibition immunoassay, including the preparation of antigen and antibody, labelling of antigen and quantitative Procedures, etc.