法医学杂志

法医学杂志 ›› 1986, Vol. 0 ›› Issue (4): 11-14.

• 论文 • 上一篇    下一篇

反向间接血凝(RPHA)法应用在血痕检验中的研究

张伟忠;周佩珩;   

  1. 浙江龙泉县卫生防疫站,浙江医科大学生化教研室,
  • 发布日期:1986-08-25 出版日期:1986-08-28

Studies on Human Blood Stain by Means of RPHA Mathod

CHANG WEIZHON; ZHOU PEIHENG DEPARTMENT OF BIOCHEMISTRY, ZHEJIANG MEDICAL UNIVERSITY   

  • Online:1986-08-25 Published:1986-08-28

被引次数

1

摘要： <正> 血痕检验目前采用的方法有肉眼观察、预试验、确证试验、种属鉴别、血型检查及出血部位检验等.用免疫法制备抗人血红蛋白(Hb)的抗血清进行人血液的法医学种属鉴别已沿用很久,但国内还未见到RPHA法应用在血痕检验中的报导.本实验室建立了该法检测人粪便隐血,并对模拟血痕的检测也作了研究.认为本法对微量血痕

Abstract: We used the RPHA method to test the human blood stain. It appers high specificity as it test with pig, cow as well as other 6 kinds of animal blood solution. The minimun quantity of detectabl human Hb is 6.48ng. Synthetic detergent does not interfer with this method. Put the sand, cloth and rusty iron with human blood in 37℃ for 15 days, there is no influence to the result. The method is simple and accurate without any special instruments and reagents.