法医学杂志

法医学杂志 ›› 1990, Vol. 0 ›› Issue (1): 28-32.

• 论文 • 上一篇    下一篇

血中先锋霉素的分离与测定

孙亚娟;吴侔天;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,
  • 发布日期:1990-02-25 出版日期:1990-02-28

SEPARATION AND DETERMINATION OF CEPHALOSPORINS IN BLOOD WITH THIN-LAYER CHROMATOGRAPH

SUN YAJUAN, WU MUTIAN INSTITUTE OF FORENSIC SCIENCES ,MINISTRY OF JUSTICE,PRC   

  • Online:1990-02-25 Published:1990-02-28

摘要： <正> 先锋霉素类药物是广泛使用的抗菌药物,有过敏反应或与青霉素有交叉过敏现象.在工作中我们曾遇到过由于注射先锋霉素过敏致死的医疗纠纷案例.使用文献所报道的高效液相色谱方法和荧光法检案时发现,检血中加入不同沉淀剂离心后直接取样进行液相色谱分析,由于药浓稀释,不易检出而且不能准确定性.荧光法不仅操作繁杂,同样难以定性.我们应用薄层色谱扫描法对几种常见先锋霉素药物的分离、定性做了一些考察,并对最常应用的头孢噻吩钠(先锋霉素1号)在血中的检测作了较为详细的探索,取得了比较满意的结果.

Abstract: In this paper,a method for separating and determinating eight cephalosporins are developed with CS-930 Dual-Wavelenth Thin-Layer Chromatograph scanner. The cephalosporins are confirmed by their Rf and UV bsorbed curves,The relative recovery of extracting cephalothin from blood with chloroform is about 90%.The linear scope of quantiting cephalothin is between 0.5 and 8.0ug. The stability of cephalothin in postmortem blood is also investigated.