法医学杂志

法医学杂志 ›› 1990, Vol. 0 ›› Issue (2): 11-15.

• 论文 • 上一篇    下一篇

荧光法测定兔死后组织中DNA含量变化

徐俊杰;阙庭志;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,
  • 发布日期:1990-04-25 出版日期:1990-04-28

In The Organism of Dead Rabbit DNA Quantity Has Changed By Fluorimetry

XU JUN JIE; QUE TINGZHI INSTITUTE OF FORENSIC SCIENES, MINISTRY OF JUSTICE, PRC   

  • Online:1990-04-25 Published:1990-04-28

被引次数

34

摘要： <正> 存在于活细胞的细胞核中的脱氧核糖核酸(DNA)是一类具有显著生化稳定性的物质;在同一物种的不同组织的细胞核中DNA含量是恒定的.当动物细胞死亡时,细胞核中染色体内的DNA会发生减少,但动物细胞的死亡是发生在动物临床死亡之后,所以当动物死亡后其DNA一般需要较长时间才自溶破

Abstract: This paper reported the method for quantitative determination of DNA by the fluorescent dye of DAPI. To make use of fluorimetry detected directly quantity of DNA in the kidenys, livers, spleens, cardiac muscles and skeletal muscles of rabbit to died by ether. And assied the change of DNA quantity following to seven hours for organism.1. The quantity of DNA was not changed following the postmortem in the kidenys, cardiac and skeletal muscles.2. In the spleens, the quantity of DNA was increased following the postmortem.3. In the livers, the quantity of DNA was deceased following the post. mortem.