法医学杂志 ›› 1990, Vol. 0 ›› Issue (2): 19-20.

• 论文 • 上一篇    下一篇

超薄PAGIEF法检测PGM1亚型

王秀玲;贾静涛;   

  1. 中国医科大学法医学系法医血清学教研室,中国医科大学法医学系法医血清学教研室,
  • 发布日期:1990-04-25 出版日期:1990-04-28

An Ultra-thin PAGIEF Technique used for Phenotyping PGM1 Subtypes

WANG XIULING; JIA JINGTAO DEPARTMENT OF FORENSIC SEROLOGY, FACULTY OF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY, SHENYANG   

  • Online:1990-04-25 Published:1990-04-28

摘要: <正> 葡糖磷酸变位酶(PGM)型是一个有重要实际意义的遗传多型性系统.自1972年Ishimoto等将聚丙烯酰胺凝胶等电点聚焦技术(PAGIEF)应用于PGMI同工酶的研究,并发现PGMI亚型以来,相继有许多学者进行了这方面的研究,他们所应用的都是凝胶厚度为1 mm的薄层PAGIEF技术;自1982年以来又有应用凝胶厚度为0.15mm的超薄层PAGIEF技术的报道、国内自王嵬等报道应用薄层技术检测PGM_1亚型以来,尚未见有应用超薄层技术进行此项检测的报道.本文将已在我室实际应用的0.1mm超薄技术报道如下.

Abstract: In this paper, we recommonded an ultra-thin PAGIEF technique which can be used for phenotyping the PGM1 subtypes. This technique may be able to save a great quantity of stock gel solution, Ampholine and staining solution, and to reduce the operating time significantly. The most samples we used were the freeze red cells reserved over one year. This indicates that the freezing method is important for reserving the reference red cells.