法医学杂志 ›› 1991, Vol. 0 ›› Issue (2): 16-19.

• 论文 • 上一篇    下一篇

固相放射免疫分析法检测ABO血型

吴奋强;郭景元;   

  1. 中山医科大学法医系物证教研室,中山医科大学法医系物证教研室,
  • 发布日期:1991-04-25 出版日期:1991-04-28

Determination of ABO Blood Groups by Solid Phase Radioimmunoassay

WU FEN-QIANG GUO JING YUAN DEPARTMENT OF FORENSIC SEROLOGY,SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES   

  • Online:1991-04-25 Published:1991-04-28

摘要: <正> 固相放射免疫分析(Solid Phase Ra-dioimmunoassay,RIA)是一种既具有抗原抗体相结合的特异性好,又具有放射性同位素的灵敏度高的优点的方法。该法在其他学科中已得到某些应用,但在法医生物学领域中尚未见报道。本实验对该法在血液的ABO 血型测定的应用作了初步探讨。

Abstract: This paper reported a very sensitive,precise and rapid method for detecting ABO blood groups.We used anti-A and anti-B McAb as the first antibodies and~(125)Ⅰ-labelled antl-mouse IgG the second anti- body.The sensitivity of this method could reach 1:5000 dilution,and the blind test of all samples were correct.These results showed that SPRIA should be practical used in identifying blood group antigens from bloodstain,saliva,semen,etc.