法医学杂志

法医学杂志 ›› 1995, Vol. 0 ›› Issue (4): 0-0.

• 论文 •    

银环蛇中毒死亡鉴定方法的实验研究(Ⅱ)──长碳臂活化生物素─马抗银环蛇抗体的制备

闫祖康，祝家镇   

  1. 上海医科大学法医学系，中山医科大学法医学系
  • 发布日期:1995-08-25 出版日期:1995-08-28

Identification of Death Caused by Bungarotoxin: (2) Synthesis of Biotinglated Horse Anti-Bungarus IgG F (ab') 2

YAN ZULCANG(FACULTY OF FORENSIC MEDICINE, SHANGHAI MEDICAL UNIVERSITY; SHANGHAI, 200032), ZHU JIAZHEN(FACULTY OF FORENSIC MEDICINE, SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES; GUANGZHOU, 510089)   

  • Online:1995-08-25 Published:1995-08-28

摘要： 作者用ｄ－生物素自制Ｎ－羟基丁二酰亚胺６－生物素化酰氨已酯（长碳臂活化生物素）。质谱分子离子峰测定分子量为４５４；红外光谱分析各官能基团与分子结构式吻合。探讨了长碳臂活化生物素与精制马抗银环蛇抗体交联的最适比例。与普通活化生物素标记、辣根过氧化物酶标记的同样抗体相比，稀释滴度分别提高４—８倍。

关键词: N—羟基丁二酰亚胺6－生物素化酰胺已酯, 生物素, 蛇咬伤鉴定

Abstract: N-hydroxysuccinimido 6-(biotinylamido) hexanoate (NHBH) prepared from d-biotin was measured with mass spectroscopy, which gave a molecular weight at 454. Further characterization by infraied spectroscopy confirmed the desired functional groups. The optimization of the molar incorporation ratios between NHBH and horse anti-Bungarus IgG (ab') 2 previously purified by affinity Chromatography is investigated. The enhancement of the dilution titer of the conjugate was 4 and 8 fold higher than that of conven tiotal biotin-theied conjugate and peroxidase conjugate, respectively.

Key words: N-hydroxysuccinimido 6-(biotinlamido) hexanoate, bungarotoxin, d-biotin, snake biting