法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    

失血即刻死亡心肌超微结构、组织化学及免疫组化研究

高彩荣，王英元，冀春萱，路健，刘育艳   

  1. 山西医学院法医病理学教研室，山西医学院病理解剖学教研室
  • 发布日期:1996-04-25 出版日期:1996-04-28

Ultrastructural histochemical and immunohistochemical study on myocardium of the rabbits in hemorrhagic sudden death

GAO CAIRONG; WANG YINGYUAN; JI CHUNXUAN; ET AL (DEPARTMENT OF FORENSIC PATHOLOGY.SHANXI MEDICAL COLLEGE;SHANXI 030001)   

  • Online:1996-04-25 Published:1996-04-28

被引次数

7

摘要： 本研究应用电镜、组织化学、免疫组化及形态计量分析等方法和技术，观察失血即刻死亡时家兔心肌超微结构、琥珀酸脱氢酶（ＳＤＨ）活性、肌红蛋白（Ｍｂ）脱失的变化特点。结果发现，失血即刻死亡时家兔心肌细胞轻度水肿，肌原纤维普遍强度收缩，肌节缩短，Ｚ线增粗，心肌细胞内ＳＤＨ活性明显下降，Ｍｂ轻度局灶性脱失．

关键词: 失血性休克, 心肌超微结构, 琥珀酸脱氢酶, 肌红蛋白

Abstract: Transmission electron microscopy (TEM), histochemistry, immunohistochemistry and morphometry were employed to study ultrastructural characteristics, succinic dehydrogenase (SDH) activity and myoglobin (Mb) content of the rabbit myocardium in hemorrhagic sudden death. The results are summarized as follows: The changes of the myocardium apperaed when hemorrhgic sudden deathmachnde mild intercellular edema of the myocytes; supercontracting of the myofibrils, shortening of the sarcomeres, widening of the z-bands;turbing in alliance of tile respective bands of the parallel arrangement of myofibrils; obviosly decreasing of SDH activity, slight focal deletion of Mb. The mechanism and the significance of these changes on forensic medicine are discussed.

Key words: hemorrhagic shock, myocardial ultrastructure, succinic dehydrogenase, myoglobin