法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (3): 0-0.

• 论文 •    

线粒体DNA测序分析及其法医学应用

郭景元，赵勇   

  1. 中山医科大学法医物证学教研室
  • 发布日期:1996-06-25 出版日期:1996-06-28

Mitochondrial DNA Sequencing Analysis and Its Application in Forensic Science

GUO JINGYUAN AND ZHAO YONGA (DEPARIMENT OF FORENSIC SCIENCE, SUN YAT-SAN UNIVERSITY OF MEDICAL SEIENCE. GUANGDONG GUANZHOU 510089)   

  • Online:1996-06-25 Published:1996-06-28

被引次数

5

摘要： 本文建立一种以γ－３２ＰＡＴＰ直接标记ＰＣＲ扩增引物为测序引物、纯化的ＰＣＲ扩增产物为模板、Ｔａｑ酶直接测序的方法、对１００倒无血缘关系的中国汉族人线粒体ＤＮＡ主要高变区Ｄ环区的３２８个碱基（第１６０９１－１６４１８位）进行了核酸序列分析，检出８６种基因型，出现率最高者为７％；检出８１个可变碱基位点。两个非血缘关系的中国汉族人出现完全相同序列的概率为０．００６８。通过对２个四代家系．５个三代家系和１０个两代家系分析扯实线粒体ＤＮＡ是母系遗传。

关键词: 线粒体DNA, D环区：多聚酶链反应（PCR）, 核酸测序

Abstract: A methods for direct-sequencing by Tag DNA polymerase has been developed Using this method, nucleotide sequences of major hypervariable region of human mtDNA from 100 unrelated Chinese Han population have been determined. The sequences all include a 328bp (from 16091 to 16418 site)fragment in D-loop-forming region. By comparison of the sequences, 86 mtDNA types were observed among these 100 individuals, the highest frequency among them was 7％. 81 variable sites were found. The probability of two unrelated individuals of Chinese Han population having same sequence in the sequencing seament of D-loop region is 0.0068. Our data showed that mtDNA is maternally inherited in 4 generations of 2 families and 3 generations of5 families and 2 generations of 10 families.

Key words: mtDNA. D-loop. PCR. nucleotide sequence.