法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (3): 0-0.

• 论文 •    

不同机械性损伤皮肤创缘EGFR表达的研究

李朝晖，祝家镇   

  1. 中山医科大学法医病理教研室
  • 发布日期:1996-06-25 出版日期:1996-06-28

Study of Distribution of EGFR in Different mechanical Injures of Skin

LI ZHAOHUI AND ZHU JIAZHEN (DEPTARTMENT OF FORENSIC MEDICINE. SUN YATSHAN UNTVERSUY OFMEDICAI SCIENCES. GUANGDONG GUANGDONG 510089)   

  • Online:1996-06-25 Published:1996-06-28

被引次数

4

摘要： 对５１例人体尸检材料及４０只ＳＤ大白鼠不同损伤时间组标本进行组织内表皮生长因子受体（ＥＧＦＲ）的酶标染色，结果发现：生前不同方式损伤类型，ＥＧＦＲ阳性染色率在不同损伤时间表达不同。刀伤、枪伤ＥＧＦＲ阳性染色率为７０．０％－８０．０％，而挫伤和索沟皮损的ＥＧＦＲ阳性染色率较低，仅为４．０％－１０．０％左右。动物实验结果提示，损伤５分钟至３０分钟；ＥＤＦＲ阳性率较低，仅为４００％，从０．５小时至２小时、阳性染色率明显提高，为６０．０％～１００．０％，我们实验还发现，ＥＧＦＲ在人体和动物死后伤均为阴性染色。故此，可用其作为法医生前和死后伤参考指标。

关键词: 表皮生长因子受体, 机械性损伤, 免疫组织化学技术

Abstract: The immunohistochemical mathod (SP) has been used to examine the distribution of EGFR in different mechanical injures of skin (51 cases human. 40 casec rat). The results showed that:(1) The positive rates of EGFR in the different types of injures are different. Incised and gunshot wound are 70.0% to 80.0%. contused wound and ligature furrow are lower than above. only about 4.0%-10.0%. (2)Staining patterns of EGFR were observed from diffenrent time groups of rats (5 min, 10 min, 15 min, 30 min, 1hr., 1.5hr, 2 hr, after injury). The positive rates expressed low at 5 min to 30min (40%) and high at 0.5 hr. to 2hr. (60.0% to 100.0%).(3) Normal skin and postmortem wound both human and rats can't be stained by EGFR.

Key words: EGFR. Mechanical injures: Immunohitochemistry