法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    

大鼠闭合性脑损伤后血清髓鞘碱性蛋白研究

于晓军,陈国弟,苟清,吴梅筠   

  1. 吉林医学院法医学教研室,华西医科大学法医学系
  • 发布日期:1999-04-25 出版日期:1999-04-28

MBP content in serum after closed diffuse cranial injury in rats

YU XIAOJUN,CHEN GUODI,GOU QING,ET AL(DEPARTMENT OF FORENSIC MEDICINE,JILIN MEDICAL COLLEGE,JILIN 132001,P.R.CHINA)   

  • Online:1999-04-25 Published:1999-04-28

被引次数

9

摘要： 用ＥＬＩＳＡ双抗体夹心方法研究了大鼠闭合性弥漫性脑损伤后，血清髓鞘碱性蛋白（ＭＢＰ）含量的时序变化。正常大鼠血清中ＭＢＰ为６．１６３３±１．５３０１ｎｇ／ｍｌ（Ｘ±Ｓ）。伤后立即死亡组大鼠的血清ＭＢＰ为１１．３８１８±２．６５７４ｎｇ／ｍｌ，伤后１５ｍｉｎ，为１０．８３１９±２．３１３５ｎｇ／ｍｌ，此血清高ＭＢＰ水平一直持续到伤后３天。伤后第４天和第５天，血清ＭＢＰ基本恢复到正常水平。立即死亡组、伤后１５ｍｉｎ组和伤后３天之内各组的ＭＢＰ水平与正常组和伤后第４天和第５天的比较，Ｐ＜０．０１。认为可进一步探讨将血清ＭＢＰ含量的检测，作为脑震荡性脑损伤的辅助生化诊断指标之一

关键词: 闭合性弥漫性脑损伤, 髓鞘碱性蛋白, ELISA双抗体夹心方法

Abstract: The MBP content change in serum was observed by ELISA in rats with experimental closed diffuse cranial injury.The MBP content was 6.1633± 1.5301ng/ml(X±S) in the normal group,11.3818±2.6574ng/ml in the first group died immediately after injury, 10.8319±2.3135ng/ml in the second group of 15 min after injury . This increased level of MBP was lasting in the following 3 days and returned to normal at the 4th and 5th day after injury. The difference of the MBP levels between the groups after injury and that in the normal group is significant (P<0.01).

Key words: closed diffuse cranial injury, myelin