法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    

武汉地区汉族人群CSF1PO座位多态性分析

陈慧,余纯应,杨荣芝,杨庆恩   

  1. 同济医科大学法医系物证教研室
  • 发布日期:1999-04-25 出版日期:1999-04-28

Polymorphic study of CSF1PO locus in Han population in Wuhan

CHEN HUI, YU CHUNYING, YANG RONGZHI, ET AL (DEPARTMENT OF FORENSIC BIOLOGY, TONGJI MEDICAL UNIVERSITY, WUHAN 430030, P.R.CHINA)   

  • Online:1999-04-25 Published:1999-04-28

被引次数

4

摘要： 应用Ｙｏｓｈｉｄａ设计的ＣＳＦ１ＰＯ新引物对武汉地区３１２例汉族个体作了分型调查，获得了武汉地区汉族人ＣＳＦ１ＰＯ座位的基因频率资料。与Ｈａｍｍｏｎｄ等提出的传统引物扩增效果相比较，本文所选用的引物更适用于法医检案，尤其对严重降解ＤＮＡ材料的分型效果有明显的优越性

关键词: 短串联重复序列（STR）, 聚合酶链反应（PCR）, 扩增片段长度多态性, CSF1PO

Abstract: Using the primers designed by Yoshida, the amplified fragment length polymorphism of the STR locus CSF1PO was studied. 8 alleles were determined from 312 unrelated individuals of Han population living in Wuhan. Sensitivity and suitability of the PCR-STR typing using Yoshida's primers were compared with the one using Hammond's. Results proved that Yoshida's method were more suitable in forensic identification than that of Hammond, especially in the typing of severely degraded DNA samples.

Key words: STR, PCR, CSF1PO locus, amplified fragment length polymorphism