法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (4): 193-195+.

• 论文 •    下一篇

急性心肌缺血再灌FOS免疫组织化学实验研究

马孟云,徐小虎,罗斌,祝家镇,陈建国,赵连旭,王海鱼   

  1. 中山医科大学法医病理学教研室!广东广州,510089,汕头大学医学院法医研究所!广东,515031,中山医科大学法医病理学教研室!广东广州,510089,中山医科大学法医病理学教研室!广东广州,510089,中山医科大学法医病理学教研室!广东广州,510089,中山医科大学法医病理学教研室!广东广州,
  • 发布日期:1999-08-25 出版日期:1999-08-28

Immunohistochemical study on myocardial acute ischemia reperfusion injury in rats with anti-FOS protein antibody

MA MENGYUN; XU XIAOHU; LUO BIN, ET AL. (DEPARTMENTOF FORENSIC PATHOLOGY, SUN YAT-SEN UNIVERSITY OF MEDICAL SCICNCES, CUANGZHOU 510089, P. R. CHINA)   

  • Online:1999-08-25 Published:1999-08-28

被引次数

3

摘要： 探寻FOS蛋白在心肌早期缺血再灌流损伤中变化规律，为心性猝死的诊断提供新方法。利用SD大鼠建立心肌缺血再灌流损伤模型，设立正常、缺血对照组与缺血再灌组。心脏标本经HE染色及免疫级化观察。结果发现，在冷冻切片上缺血20min再灌流30min，再灌流区心肌细胞核呈阳性着色。但在石蜡切片上，缺血30min再灌流30min后，再灌流区才有心肌细胞核（37．76％±9．66％）呈弱阳性着色，再灌流60min后核呈棕褐色阳性染色，120min后开始减弱（35.36％±3.16％）。正常和单纯缺血组心肌细胞核未见有阳性反应。HE染色无明显病理改变。结果提示，SABC－FOS免疫组织化学方法最早可揭示心肌缺血20min再灌流30min的损伤，FOS蛋白在再灌流后60－120min之间可能有一个高峰表达。此法对显示实验性心肌早期缺血再灌流损伤有重要的价值。有望用于心性猝死的诊断。

关键词: 心肌急性缺血再灌, FOS蛋白, 免疫组化

Abstract: To investigate law of FOS protein induced by myocardial ischemia reperfusion (MI/R) in acute period, amodel in 20 anaethetized SD rats was established. Ratswith normal and ischemia were used as control groups.Specimens were studied immunohistochemically with c fos antibody. Afer ischemia 20 minutes, followed by 30minutes reperfusion, the area of MI/R showed nuclei ofmyocytes positive staining in cryosection slides. In C2group, the area showed nuclei of myocytes (37. 76%±9. 66% ) positive staining weakly. In C3 group, nuclei ofcardiac myocytes(40. 34% ± 3. 32% ) was significantlypositive. In C4 group it began to attenuate(35. 36% ±4. 81% ). The myocardium in normal and ischemic control groups showed negative staining. No changes wereseen with HE staining. Our data indicated that immunohistochernical method may reveal acute MI/R injury ofischemia 20 min and reperfusion 30 min with anti - FOSprotein staining and there is problely a peak between 60 -120min after reperfusion. It is possible that the method beused to diagnose sudden cardiac death in forensicmedicine.

Key words: Myocardial acute ischemia reperfusion, immunohistochemical study, FOS protein