法医学杂志

法医学杂志 ›› 2000, Vol. 0 ›› Issue (3): 141-142+.

• 论文 • 上一篇    下一篇

大鼠心肌早期缺血内皮素的表达

马孟云,徐小虎,罗斌,吴晓华,祝家镇   

  1. 中国人民公安大学侦察系法医学教研室!北京100038,汕头大学医学院法医学教研室!广东515031,中山医科大学法医病理学教研室!广东广州510089,汕头大学医学院法医学教研室!广东515031,中山医科大学法医病理学教研室!广东广州510089
  • 发布日期:2000-06-25 出版日期:2000-06-28

Expression of ET of early ischemic myocardium in rats

MA MENGYUN,ET AL (SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES,GUANGZHOU 510089,PRC)   

  • Online:2000-06-25 Published:2000-06-28

被引次数

5

摘要： 为探讨心肌缺血后内皮素在心肌组织内表达的变化。用免疫组化SABC法研究内皮素的表达情况。结果发现缺血60min后 ,缺血区心肌细胞呈局灶性阳性 ;缺血120min后 ,血管平滑肌、内皮细胞及心肌细胞浆均呈阳性着色。对照和缺血30min组心肌组织未见有阳性反应。上述各组HE染色未见明显病理改变。

关键词: 早期心肌缺血, 内皮素, 免疫组化, SABC法

Abstract: To study the expression of ET of early ischemic myocardium, we designed a model of early myocardial ischemia (EMI) in rats The study was conducted with SABC method and HE staining Cardiac myocytes of ischemia showed a little ET-positive at 60-min Cardiac myocytes, endothelium and smooth muscle of vessel showed ET-positive staining in the area of EMI after 120-min Staining was negative for Ischemia myocardium at 30-min and for the control group No change was seen with HE staining in all groups The data indicates that ET-SABC method may reveal the injury of myocardial ischemia 60-min and longer and may provide a new marker to diagnose EMI in forensic pathology

Key words: Early myocardial ischemia, ET, ABC method(Original article on page 141)