法医学杂志

法医学杂志 ›› 2002, Vol. 0 ›› Issue (1): 22-25.

• 论文 • 上一篇    下一篇

尿样中苯骈二氮杂类药物筛选分析研究

沈保华,沈敏,卓先义,赵子琴,吴何坚,卜俊   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,复旦大学基础医学院法医教研室,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063 ,上海200063 ,上海200063 ,上海200032 ,上海200063 ,上海200063
  • 发布日期:2002-02-25 出版日期:2002-02-28

Screening of benzodizepines and their metabolites in urine

SHEN BAO-HUA,SHEN MIN,ZHUO XIAN-YI,ET AL.( INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, SHANGHAI 200063)   

  • Online:2002-02-25 Published:2002-02-28

摘要： 目的建立尿样中苯骈二氮杂类药物两种筛选分析方法。方法用GC/ECD、GC/MS直接测定苯骈二氮杂类药物原体和GC/ECD、GC/MS测定1,4-苯骈二氮杂类药物的酸水解产物苯甲酮同系物。结果GC/ECD直接测定苯骈二氮杂类药物方法,大部分药物的回收率为60%～90%,线性范围为20～200ng/ml尿,线性相关系数大于0.99,最低检出限达0.5ng/ml～10ng/ml。结论所建两种方法各有其特点又可相互补充,已成功地应用于司法鉴定实践。

关键词: 苯骈二氮杂类药物, 苯甲酮类同系物, GC/ECD, GC/MS

Abstract: Objective Screen seventeen benzodizepines and their metabolitesin urine by GC/ECDand GC/MS . Methods They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines. Results The methods were simple and sensitive. The recoverys were 60%to 90%of most benzodizepines, linear calibration curves were 20ng/ml-200ng/ml(r >0.99), and detection limits were 0.5ng/ml-10ng/ml. Conclusion They methods were evaluated with human urine samples.

Key words: benzodizepines, benzophenones, GC/ECD, GC/MS