法医学杂志

法医学杂志 ›› 2002, Vol. 0 ›› Issue (3): 152-154.

• 论文 • 上一篇    下一篇

应用PCR-SSCP技术检测PGM_1基因型

宋海燕,杨庆恩,余纯应   

  1. 华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系 湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030
  • 发布日期:2002-06-25 出版日期:2002-06-28

PGM_1 genotyping by PCR-SSCP

SONG HAI-YAN,YANG QING-EN,YU CHUN-YING(DEPARTMENT OF FORENSIC MEDICINE,TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN430030)   

  • Online:2002-06-25 Published:2002-06-28

摘要： 目的应用PCR-SSCP技术分型PGM1基因型。方法提取156份武汉地区汉族无关个体的血样DNA,分别扩增PGM1基因外显子4和外显子8的多态性靶DNA,用SSCP分析PCR产物,判断基因型。结果两种PCR产物均检出了两个等位基因、三种基因型,DP值分别为0.5620、0.4405。综合外显子4和8的PCR-SSCP结果,分出8种PGM1基因型,DP值为0.7318。应用本法对保存10年的陈旧血痕和精斑PGM1分型成功。结论用PCR-SSCP分型PGM1基因型在法医物证检验中具有实用价值。

关键词: 磷酸葡萄糖变位酶1(PGM1), 基因型, PCR-SSCP

Abstract: Objective PGM 1 genotyping by PCR-SSCP analysis.Methods Amplified genome DNA from156unrelated Han individuals living in Wuhan,PCR products for exon4and exon8of PGM 1 were then analyzed by SSCP to detect the genotypes.Results2alleles and3genotypes were detected in exon4and8respectively.The discrimination power was0.7318.PCR-SSCP analysis was suitable for determination of PGM 1 genotypes from old blood and semen stains.Conlusion PGM 1 system typed by PCR-SSCP is useful for forensic identification.

Key words: PGM 1, genotype, PCR-SSCP