法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (3): 155-159.

• 论文 • 上一篇    下一篇

烧骨DNA检验技术的研究

叶健,季安全,赵兴春,涂政,张志强,朱世峰,袁丽   

  1. 公安部第二研究所,公安部第二研究所,公安部第二研究所,公安部第二研究所,菏泽市公安局,菏泽市公安局,中国人民公安大学 北京100038 ,北京100038 ,北京100038 ,北京100038 ,山东荷泽274039 ,山东荷泽274039 ,北京100038
  • 发布日期:2004-06-25 出版日期:2004-06-28

Study of DNA identification in burned bones

YE JIAN,JI AN-QUAN,ZHAO XING-CHUN,ET AL.(INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF PUBLIC SECURITY,BEIJING100038,CHINA)   

  • Online:2004-06-25 Published:2004-06-28

被引次数

30

摘要： 目的解决陈旧性骨骼和烧骨DNA检验难题。方法研究建立了CTAB法裂解提取DNA,再用磁珠纯化得到的DNA提取液进行STR复合扩增检验。结果实验结果及实际检案显示研究所建立的骨DNA提取方法能较好地去除DNA扩增抑制物,得到高质量的DNA模板。结论本研究所建立的烧骨DNA检验方法其识别率为10×10-12,达到个人同一认定的目的,在解决实际工作中杀人焚尸案、火灾、爆炸等恶性案件和事故中有重要的作用。

关键词: 十六烷基三甲基溴化胺(CTAB), 陈旧性骨骼和烧骨DNA, 磁珠纯化, 法医DNA检验, 荧光标记STR复合扩增

Abstract: Objective For the purpose of solving a problems of DNA testing of burned bones.Methods We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammoni-um bromide(CTAB)lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purifi-cation using the DNA IQTMSystem.Results The methods were found to be effective in removing the PCR inhibitors from the burned bone.Then the extracted DNA was successfully genotyped by using the flores-cence labeling STR multiplex method.Conclusion The results of this research will assist forensic scien-tists in the identification of DNA from victims whose bodies underwent significant trauma or burning,pre-cluding the utilization of traditional forensic DNA identification techniques.

Key words: cetyltrimethyl ammonium bromide(CTAB), collagen aged bones and burned bones DNA, DNA IQTMsystem, purification, forensic DNA examination, florescence labeling STR multiplex