大鼠皮肤挫伤后Beclin1和LC3的表达

法医学杂志 ›› 2007, Vol. 0 ›› Issue (1): 11-13+8.

大鼠皮肤挫伤后Beclin1和LC3的表达

颜峰平;陈忆九;黄晓华;

复旦大学上海医学院法医学系,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所上海200032,司法部司法鉴定科学技术研究所,上海200063,上海200063,上海200063

发布日期:2007-02-25 出版日期:2007-02-28

Expression of Beclin1 and LC3 after rat′s Skin Contusion

YAN FENG-PING1,2,CHEN YI-JIU2,HUANG XIAO-HUA2(1.DEPARTMENT OF FORENSIC MEDICINE,SHANGHAI MEDICAL COLLEGE,FUDAN UNIVERSITY,SHANGHAI 200032,CHINA;2.INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA)

Online:2007-02-25 Published:2007-02-28

摘要/Abstract

摘要： 目的研究大鼠皮肤损伤后Beclin1、LC3的表达,观察皮肤损伤中的自噬现象,并探讨其在损伤诊断及损伤经历时间推断的意义。方法运用免疫组化及图像分析技术研究大鼠皮肤挫伤后、损伤不同时间段后Beclin1、LC3表达的变化。结果大鼠正常皮肤表皮细胞Beclin1、LC3低表达,大鼠皮肤挫伤6h后局部表皮细胞Beclin1、LC3表达开始增多,挫伤3、5和7d呈强表达。结论皮肤损伤后局部细胞Beclin1、LC3表达增多;其表达与损伤经历时间具有相关性;细胞自噬增强是对损伤的反应。

关键词: 挫伤, 自噬, Beclin1, LC3, 免疫组化

Abstract: Objective This study aimed at examining the expression of Beclin1 and LC3 in the skin of rat after contusive injury and at observing the process of autophagy in response to injury or trauma.Their significance in diagnosis of trauma and application in estimation of the post injury time interval were also investigated.Methods Immunohistochemistry and imaging analysis were used to examine the expression level of Beclin1 and LC3 at the injury site of the rat skin at different post-traumatic times.Results Expression of Beclin1 and LC3 in normal rat skin or immediately after injury maintained at a very low level,but their level started to elevate 6 hours after injury and showed increasing strong expression at post injury time of day 3,day 5 and day 7.Conclusion Over-expression of Beclin1 and LC3 as well as increased autophagy,a response to injury,can be observed in the rat skin at the injury site.The expression level of Beclin1 and LC3 starts to increase 6 hours and shows a linear increase up to 7 days after injury.Beclin1 and LC3 may serve as a marker for estimation of the post injury time interval.

Key words: contusion, autophagy, Beclin1, LC3, immunohistochemistry

颜峰平;陈忆九;黄晓华;. 大鼠皮肤挫伤后Beclin1和LC3的表达[J]. 法医学杂志, 2007, 0(1): 11-13+8.

YAN FENG-PING;CHEN YI-JIU;HUANG XIAO-HUA(.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA). Expression of Beclin1 and LC3 after rat′s Skin Contusion[J]. , 2007, 0(1): 11-13+8.

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