法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (6): 424-427.

• 论文 • 上一篇    下一篇

二醋吗啡对大鼠心肌细胞游离Ca~(2+)浓度的作用

刘小山;吴敬东;蒿自睿;刘水平;李朝晖;李海霞;陈玉川;   

  1. 中山大学中山医学院法医系,中山市公安局石歧分局,中山大学中山医学院法医系,中山大学中山医学院法医系,中山大学中山医学院法医系,中山大学中山医学院法医系,中山大学中山医学院法医系 广东广州510080,广东中山528403,广东广州510080,广东广州510080,广东广州510080,广东广州510080,广东广州510080
  • 发布日期:2007-12-25 出版日期:2007-12-28

The Effects of Heroin on Intracellular Free Ca~(2+) of Rat Myocardium

LIU XIAO-SHAN1, WU JING-DONG2, HAO ZI-RUI1, LIU SHUI-PING1, LI ZHAO-HUI1, LI HAI-XIA1, CHEN YU-CHUAN1 (1. DEPARTMENT OF FORENSIC MEDICINE, MEDICAL COLLEGE OF SUN YAT-SEN UNIVERSITY, GUANGZHOU 510080,CHINA; 2.SHIQI BRANCH OF PUBLIC SECURITY BUREAU OF ZHONG   

  • Online:2007-12-25 Published:2007-12-28

摘要： 目的观察二醋吗啡对心肌细胞内游离Ca2+浓度的作用。方法取自1～3d的SD大鼠培养的心肌细胞,用荧光探针Fluo-3/AM负载心肌细胞,不同浓度及不同剂量的二醋吗啡作用心肌细胞,激光共聚焦显微镜下检测Ca2+的变化。结果不同剂量及浓度的二醋吗啡对心肌细胞内游离Ca2+浓度有不同作用,一定浓度的二醋吗啡使心肌细胞内游离Ca2+浓度呈剂量依赖性的升高、短时间内心肌细胞[Ca2+]i荧光强度增强,并产生[Ca2+]i峰。结论探索出二醋吗啡导致心肌细胞内Ca2+变化的有效浓度,为进一步深入研究打下了基础。

关键词: 二醋吗啡, 心肌细胞, 游离Ca2+

Abstract: Objective To observe the effects of heroin on intracellular free Ca2+ in rat myocardium. Methods The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope. Results Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2+concentration) leading to [Ca2+]i peak. Conclusion Heroin could affect concentrations of [Ca2+]i in myocardium and its dosage related effect needs further investigation.

Key words: heroin, myocardial cells, free, Ca2+