Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology

doi:10.3969/j.issn.1004-5619.2015.02.001

法医学杂志 ›› 2015, Vol. 31 ›› Issue (2): 81-84.DOI: 10.3969/j.issn.1004-5619.2015.02.001

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Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology

发布日期:2015-04-25 出版日期:2015-04-28

Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology

DU QIU-XIANG1, WANG XIAO-WEI1,2, ZHANG LEI1,2, LI SAN-QIANG1, GAO CAI-RONG1, WANG YING-YUAN1, SUN JUN-HONG1

（1. Department of Forensic Pathology, Shanxi Medical University, Taiyuan 030001, China; 2. Criminal Police Department of Chongqing Public Security Bureau, Chongqing 400021, China）

Online:2015-04-25 Published:2015-04-28
Contact: SUN Jun-hong, Ph.D, associate professor, major in forensic pathology; E-mail: junhong.sun@sxmu.edu.cn
About author:DU Qiu-xiang （1982—）, Ph.D, lecturer, major in forensic pathology; E-mail: dqx_2008@126.com
Supported by:
supported by the National Science Foundation for Young Scientists of China （81001347） and the Innovation Foundation of Shanxi Medical University （01201409）

摘要/Abstract

Abstract: Objective In order to understand which kind of function genes play an important role for estimating wound age, the variation of difference genes’ mRNA expression were compared after injury. Methods The mRNA expression levels of seven candidate genes （ICAM-1, NF-κB, MX2, MT1, MT2, sTnI, and Cox6c） were analyzed in contused rat skeletal muscle at different time points using real-time fluorescent quantitative PCR （RT-qPCR）. The raw Ct values were normalized relative to that of RPL32 mRNA, and converted to standard Ct values. At each time point after injury, the standard deviations （SD） of the standard Ct values were calculated by SPSS. Results The expression trends of the seven genes were all found to be related to wound age, but there were lower variation coefficients and greater reliability of sTnI and Cox6c when compared with other genes. Conclusion The genes encoding structural proteins or proteins that perform basic functions can be suitable for wound age estimation.

Key words: forensic pathology, wounds and injuries, muscle, skeletal, rats

中图分类号:

DF795.1

DU Qiu-xiang, WANG Xiao-wei, ZHANG Lei, et al.. Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology[J]. 法医学杂志, 2015, 31(2): 81-84.

DU QIU-XIANG, WANG XIAO-WEI, ZHANG LEI, ET AL.. Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology[J]. , 2015, 31(2): 81-84.

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Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology

Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology

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