法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (2): 111-113,119.DOI: 10.3969/j.issn.1004-5619.2018.02.001

• 论著 •    下一篇

淡水溺死大鼠肺组织与血清中IL-1α、IL-1β和IL-13 mRNA表达

姜美玲1，刘志杰2，潘  静3，路  健2，梁新华2   

  1. 1. 吉林鸣正司法鉴定中心，吉林 吉林 132011； 2. 山西医科大学法医学院，山西 太原 030001； 3. 赤峰市林西县公安局，内蒙古 赤峰 025250
  • 发布日期:2018-04-25 出版日期:2018-04-28
  • 通讯作者: 梁新华，男，硕士，副教授，主要从事法医病理学与法医临床学教学与科研；E-mail：liangxhlemon@163.com
  • 作者简介:姜美玲（1988—），女，硕士研究生，主要从事法医病理学研究；E-mail：362168949@qq.com
  • 基金资助:
     

Expressions of IL-1α, IL-1β and IL-13 mRNA in Lung Tissue and Serum of Rats Drown in Fresh Water

JIANG Mei-ling1, LIU Zhi-jie2, PAN Jing3, LU Jian2, LIANG Xin-hua2   

  1. 1. Mingzheng Forensic Identification Centre of Jilin, Jilin 132011, China; 2. College of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 3. Linxi County Public Security Bureau of Chifeng City, Chifeng 025250, China
  • Online:2018-04-25 Published:2018-04-28
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摘要： 目的 检测溺死大鼠肺组织和血清中白细胞介素（interleukin，IL）-1α、IL-1β、IL-13 mRNA的变化，探讨其在法医学溺死诊断鉴定中的潜在价值。 方法 18只SD大鼠随机分为溺死组、空白对照组和心肌梗死组（对照）。分别收集各组大鼠右心室血清及右肺下叶、心肌，应用TaqMan探针法检测肺组织及右心室血清IL-1α、IL-1β、IL-13 mRNA的表达情况。 结果 与空白对照组及心肌梗死组比较，溺死组大鼠肺组织IL-1α、IL-1β及IL-13 mRNA表达差异均无统计学意义（P＞0.05），右心室血清IL-1β、IL-13 mRNA表达升高（P＜0.05）。空白对照组与心肌梗死组比较，血清中IL-1α、IL-1β及IL-13 mRNA表达差异均无统计学意义（P＞0.05）。 结论 细胞因子IL-1β及IL-13 mRNA在溺死后大鼠右心室血清中表达量均有改变，表明其变化与溺死密切相关。

 

关键词: 法医病理学；溺水；淡水；白细胞介素-1&alpha, ；白细胞介素-1&beta, ；白细胞介素-13；大鼠

Abstract: Objective To detect the changes of （interleukin, IL） -1α, IL-1β and IL-13 mRNA in lung tissue and serum of drown rats, and to explore the potential value for the diagnosis of drowning in forensic practice. Methods Eighteen SD rats were randomly divided into drowning group, blank control group and myocardial infarction group （as control group）. The serum of right ventricular, the inferior lobe of right lung and the myocardium were taken from the rats in different groups. The expressions of IL-1α, IL-1β and IL-13 mRNA in the lung tissue and the serum of right ventricular were detected by TaqMan probe method. Results The expression differences of IL-1α, IL-1β and IL-13 mRNA in lung tissue between drowning group and blank control group, myocardial infarction group were not statistically significant （P>0.05）. The expression of IL-1β and IL-13 mRNA in serum of right ventricular increased （P<0.05）. The expression differences of IL-1α, IL-1β and IL-13 mRNA in serum between blank control group and myocardial infarction group were not statistically significant （P>0.05）. Conclusion The changes of cytokines IL-1β and IL-13 mRNA in the serum of right ventricular of drown rats are statistical significance, which are highly correlated with drowning.

Key words: forensic pathology, drowning, fresh water, interleukin-1 alpha, interleukin-1 beta, interleukin-13, rats

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