法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (4): 252-255.DOI: 10.3969/j.issn.1004-5619.2012.04.003

• 论著 • 上一篇    下一篇

扩张型心肌病心肌Fas蛋白表达

魏淑荣1，2，陈新山1，陈煌峰1，3，孙许朋1，4，黄光照1   

  1. 1. 华中科技大学同济医学院法医学系，湖北 武汉 430030； 2. 益阳市公安局刑侦支队，湖南 益阳 413000； 3. 武汉市公安局刑事侦查局，湖北 武汉 430019； 4. 新乡医学院法医学教研室，河南 新乡 453003
  • 发布日期:2012-08-25 出版日期:2012-08-28
  • 通讯作者: 陈新山，男，博士，教授，博士研究生导师，主要从事法医病理学教学、科研和检案工作；E-mail：xschen@mails.tjmu.edu.cn
  • 作者简介:魏淑荣（1983—），女，湖北武汉人，硕士研究生，主要从事法医病理和法医物证的检案和科研工作；E-mail：lianxi19831225@yahoo.com.cn
  • 基金资助:

    武汉市科技攻关计划项目（201060623254）

Expression of Fas Protein of Myocardium in Dilated Cardiomyopathy

WEI SHU-RONG1,2, CHEN XIN-SHAN1, CHEN HUANG-FENG1,3, SUN XU-PENG1,4, HUANG GUANG-ZHAO1   

  1. 1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2. Criminal Investigation Detachment, Yiyang Public Security Bureau, Yiyang 413000, China; 3. Department of Criminal Investigation, Wuhan Public Security Bureau, Wuhan 430019, China; 4. Department of Forensic Medicine, Xinxiang Medical College, Xinxiang 453003, China
  • Online:2012-08-25 Published:2012-08-28

被引次数

3

摘要： 目的 探讨扩张型心肌病（dilated cardiomyopathy，DCM）心肌Fas蛋白的表达情况及其与DCM猝死的关系。 方法 用免疫组织化学法和图像分析技术定量检测本教研室1997—2007年间9例DCM猝死和11例暴力死或其他死者的心肌Fas蛋白表达情况。 结果 DCM猝死组心肌Fas蛋白表达明显增多，阳性着色在心肌纵切面细胞膜上及胞浆内呈棕黄色颗粒状或条状分布，横断面细胞膜见环形棕黄色颗粒带；对照组未见明显变化或仅见小面积浅淡着色。定量检测结果显示其表达面积在两组间的差异有统计学意义（P=0.002），表达的平均光密度差异无统计学意义（P=0.675）。 结论 DCM猝死者心肌Fas蛋白表达明显增多，可能与DCM患者发生的因心律失常和心功能衰竭所致猝死有关。

关键词: 法医病理学；心肌病, 扩张型；免疫组织化学；Fas蛋白

Abstract: Objective To investigate Fas protein expression of the myocardium in dilated cardiomyopathy （DCM） and its relationship with occurrence of sudden death caused by DCM. Methods Nine autopsy cases of sudden death caused by DCM along with the heart samples were chosen from the archives in the Department of Forensic Medicine, Tongji Medical College, HUST from 1997 to 2007. Other 11 cases which died of violence and other diseases were selected as the control group. Expressions of myocardial Fas protein in the samples were quantitatively detected by immunohistochemistry and computerized imaging analysis. Results Myocardial Fas protein expression increased significantly in the DCM group. Positive color showed brown-yellow granulated or striped distribution in the longitudinal section of myocardial within the cell membrane and cytoplasm, and showed circular brown granules in the cross section of the cell membrane, while these changes were not observed in the control group though there was focal weak staining noted. Statistical significance was observed between the experimental and control groups（P=0.002）, but no statistical significance was found for the average optical density value between these two groups（P=0.675）. Conclusion The expression of Fas protein increased obviously in the DCM group. Such alteration in expression quantity and distribution of myocardial Fas protein may be related to arrhythmia and heart failure in the patients with DCM.

Key words: forensic pathology, cardiomyopathy, dilated, immunohistochemistry, Fas protein

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