法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (6): 406-409.DOI: 10.3969/j.issn.1004-5619.2016.06.002

• 论著 • 上一篇    下一篇

急性乙醇中毒大鼠脑皮质α-syn表达与神经细胞凋亡的关系

李  凡1，张  越1，2，马书玲1   

  1. （1. 河南科技大学法医学院，河南 洛阳 471003； 2. 南阳市公安局新华分局，河南 南阳 473000）
  • 发布日期:2016-12-25 出版日期:2016-12-28
  • 作者简介:李凡（1964—），男，博士，教授，主要从事法医病理学和法医毒理学教学和研究；E-mail：fanlfy@mail.haust.edu.cn
  • 基金资助:

    河南省教育厅科技重点研究项目（12B330001）

Relationship between the Expression of α-syn and Neuronal Apoptosis in Brain Cortex of Acute Alcoholism Rats

LI FAN1, ZHANG YUE1,2, MA SHU-LING1   

  1. （1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, China; 2. Xinhua Branch of Nanyang Public Security Bureau, Nanyan 473000, China）
  • Online:2016-12-25 Published:2016-12-28

摘要： 目的 观察急性乙醇中毒大鼠脑皮质α-突触核蛋白（α-synuclein，α-syn）表达及神经细胞凋亡的变化，探讨乙醇对神经细胞损害的机制。 方法 用50%乙醇溶液灌胃方法制作大鼠急性乙醇中毒模型。采用免疫组织化学染色技术和图像分析方法观察大鼠急性乙醇中毒后1 h、3 h、6 h、12 h脑皮质内α-syn和caspase-3的表达。测定阳性细胞数和平均光密度值，分析其变化趋势，并进行方差分析和t检验。 结果 急性乙醇中毒组大鼠脑皮质α-syn染色阳性细胞数和平均光密度值均呈上升趋势，6 h达到峰值，12 h略有下降，但仍高于1 h、3 h组。中毒后12 h以内，大鼠脑皮质的caspase-3阳性细胞数和平均光密度值均呈逐渐上升趋势。 结论 急性乙醇中毒后脑水肿、缺氧引起α-syn异常聚集可能参与乙醇中毒后早期神经细胞凋亡过程。

关键词: 法医病理学；法医毒理学；急性乙醇中毒；&alpha, 突触核蛋白；caspase-3；细胞凋亡；脑皮质；大鼠

Abstract: Objective To observe the changes of expression of α-synuclein （α-syn） and neuronal apoptosis in brain cortex of acute alcoholism rats and to explore the mechanism of the damage caused by ethanol to the neurons. Methods The model of acute alcoholism rat was established by 50% alcohol gavage. The α-syn and caspase-3 were detected by immunohistochemical staining and imaging analysis at 1 h, 3 h, 6 h and 12 h after acute alcoholism. The number of positive cell and mean of optical density were detected and the trend change was analyzed. The variance analysis and t-test were also performed. Results The number of α-syn positive cell and average optical density in brain cortex of acute alcoholism rat increased significantly and peaked at 6 hour with a following slight decrease at 12 h, but still higher than the groups at 1 h and 3 h. Within 12 hours after poisoning, the number of caspase-3 positive cell and average optical density in brain cortex of rats gradually increased. Conclusion The abnormal aggregation of α-syn caused by brain edema and hypoxia may participate the early stage of neuronal apoptosis in brain cortex after acute alcoholism.

Key words: forensic pathology, forensic toxicology, alcoholic intoxication, alpha-synuclein, caspase-3, apoptosis, cerebral cortex, rats

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