法医学杂志

法医学杂志 ›› 1989, Vol. 0 ›› Issue (2): 1-5+10.

• 论文 •    下一篇

中国广东地区人群血清转铁蛋白遗传多态现象及其法医学应用初步探讨

郭大玮;郭景元;   

  1. 中山医科大学法医系物证教研组,中山医科大学法医系物证教研组,
  • 发布日期:1989-04-25 出版日期:1989-04-28

Human Serum Transferrin Polymorphism in Population Of Guangdong, China and Its Application in Forensic Science

GUO DAWEI GUO JINGYUAN (DEPARTMENT OF FORENSIC SEROLOGY; SUN YAN-SEN UNIVERSITY OF MEDICAL SCIENCES)   

  • Online:1989-04-25 Published:1989-04-28

摘要： <正> 人血清转铁蛋白是存于人血浆中具有遗传多态性的蛋白质、由肝脏合成;血清含量为200～300mg/100ml。转铁蛋白有运输铁及抗感染等功能;分子量为79570道尔顿,一级结构含679个氨基酸残基,等电点5.5,含糖量6％,基因定位于3q~(21)～q~(26)。转铁蛋白首先由Smithies (1957)报导。用淀粉凝胶及聚丙烯酰胺凝胶电泳可分为TfB、TfC、TfD三种主要变异型,其中TfB、TfD又可细分为20余种变异型,1978年,kuhnl和Spielmann与Thy

Abstract: The distribution of transferrin phenotypes in the population of Guangdong, China, was investigated by isoelectric focusing on polyacrylamide gel. In our Samples (n=258), the frequencies of five phenotypes were as follows: TfC1C1=56.20%, TfC2C1=31.39%, TfC2C2=5.43%, TfC1D=5.43% and TfC1B=1.55%. The allele frequencies were calculated: TFC1=0.7539, TFC2=0.2112, TfD=0.0271 and TfB=0.0078. The observed and expected phenotypes provided a good fit to Hardy-Weinberg equelibrium. The analysis of 6 mother-child pairs was in agreement with Mendelian inheritance. The phenotypes of TfC1C1, TfC2C1 and TfC2C2 were compared to those found in other populations. Based on the data, the probability of excluding pater-ity and disciminating power (DP) were 0.1803 and 0.5795, respectely. The Tf phenotypes of bloodstains can be possibly typed at 4℃ for uP to four weeks, 11°—17° for up to five days and 37℃ for up to two days.