法医学杂志 ›› 1989, Vol. 0 ›› Issue (3): 18-22+4.

• 论文 • 上一篇    下一篇

抗人精液血清特异性的研究

侯一平;吴梅筠;   

  1. 华西医科大学法医血清学教研室,华西医科大学法医血清学教研室,
  • 发布日期:1989-06-25 出版日期:1989-06-28

Study on Specificity of Anti-Human Semen Sera

HOU YIPING WU MEIYUN(DEPARTMENT OF FORENSIC SEROLOGY, WEST CHINA UNIVERSITY OF MEDICAL SCIENCES)   

  • Online:1989-06-25 Published:1989-06-28

摘要: <正> 确证精斑常用抗人精液血清作沉淀反应。这种抗血清特异性差,常与人类某些体液发生交叉反应,以致精斑确证试验结果不可靠,影响鉴定结果的准确性。作者用人类精子、精浆、精液与人类精浆特异性抗原p30分别免疫家兔,制备各种抗血清,用环状沉淀、琼脂双向扩散及免疫电泳等技术对这些抗血清的特异性进行了研究,报道如下。

Abstract: Anti-human semen sera were prepared in rabbits immunized with washed human spermatozoa, seminal plasma, whole semen and seminal plasma specific antigen p30, respectively. The specificity of the anti-sera was identified by the ring precipitation, the double immunodiffusion and the immunoelectrophoresis. The results showed that anti-p30 serum was specific, while other three kinds of anti-sera were not specific for human semen until they were absorbed with the mixture of human colostrum, serum, and saliva.