Abstract: We examined the Hp subtypes both in the human sera and in the dried bloodstains using a method for Hp purifacation, the hydrolysis of Hp performed using the β-merca- ptoethanol-ureaiodacetamid mixture and the isoelectric focusing electrophoresis on slab polyacrylamide gel. Hp phenotyping of 404 serum samples from unrelated chinese blood donors in Xian were carried out. The distribution of the Hp subtypes are: Hp~(2SS)0.25%, Hp~2F~S49.5%, Hp~(1S)6.68%, Hp~(2fS-2SS)4.21%, Hp~(2SS)-~(1S)1.49%, Hp~(2fs)-~(1S)35.89%, Hp~(2fS)-~(1S)0.495%, Hp~2-~S-~(2V)0.743%, Hp~(1S)-~(1f)0.25% and Hp~(2V)-~(1S)0.495%. The gene frequencies respectively are: Hp~(1f)0.0037, Hp~(1S)0.2574, Hp~(2fS)0.0309, Hp~(2fS)0.7017 and Hp~(2V)0.0063. The observed and expected values provided a good fit to the Hardy-Weinberg equilibrium(p>0.05). The probab ility of excluding paternity means 0.2601, and discrimination probability means 0.62. The 24 samples of bloodstains were kept in room tempreture for seven days, 4℃ for seven days and -30℃ for thirty days. In addition to three samples, the others could besubtyped agreeing with the subtype of Hp in their serum.