用双抗夹心间接ELISA方法检测p30确证人类精斑

法医学杂志 ›› 1991, Vol. 0 ›› Issue (3): 10-13.

用双抗夹心间接ELISA方法检测p30确证人类精斑

苟清;侯一平;毛咏秋;吴梅筠;

华西医科大学法医学系,华西医科大学法医学系,华西医科大学法医学系,华西医科大学法医学系,

发布日期:1991-06-25 出版日期:1991-06-28

Determination of Human Seminal Stains by Sandwich Indirect ELISA

GOU QING HOU YIPING MAO YONGQIOU WU MEIYUNDEPARTMENT OF FORENSIC SEROLOGYWEST CHINA UNIVERSITY OF MEDICAL SCIENCES

Online:1991-06-25 Published:1991-06-28

摘要/Abstract

摘要： <正> 检测p30确证精斑有下列几种方法:免疫扩散,免疫电泳,交叉免疫电泳,胶乳凝集抑制试验,薄层免疫分析和酶联免疫吸附试验(ELISA).这些方法各有优缺点,就灵敏度而论,则以

Abstract: For the purpose of confirming the human semen stains, a sandwich indirect ELISA test was established using the rabbite anti-human p30 serum and goat antihuman p30 serum. This method is sensitive enough to detect 200ng/ml p30. A blind test to examine 126 biological stains which have boon kept at room temperature for one year was carried out using this method.The test results showed 100% accuracy.There were not any false-positive and false-negative results.

Key words: Enzyme-linked immunosorbent assay Human prostatespecific antigen p30 Conclusive test of semen stains The Project Supported by National Science Foundation of China

苟清;侯一平;毛咏秋;吴梅筠;. 用双抗夹心间接ELISA方法检测p30确证人类精斑[J]. 法医学杂志, 1991, 0(3): 10-13.

GOU QING HOU YIPING MAO YONGQIOU WU MEIYUNDEPARTMENT OF FORENSIC SEROLOGYWEST CHINA UNIVERSITY OF MEDICAL SCIENCES. Determination of Human Seminal Stains by Sandwich Indirect ELISA[J]. , 1991, 0(3): 10-13.