单克隆抗体酶联免疫吸附抑制试验测定精斑G2m(n)因子

法医学杂志 ›› 1991, Vol. 0 ›› Issue (3): 6-9.

单克隆抗体酶联免疫吸附抑制试验测定精斑G2m(n)因子

侯一平;苟清;吴梅筠;

华西医科大学法医学系,华西医科大学法医学系,华西医科大学法医学系,

发布日期:1991-06-25 出版日期:1991-06-28

G2M(N) Typing in Human Semen Stains by ELISA-I Test with Monoclonal Antibody

HOU YIPING GOU QING WU MEIYUNDEPARTMENT OF FORENSIC SEROLOGYWEST CHINA UNIVERSITY OF MEDICAL SCIENCES

Online:1991-06-25 Published:1991-06-28

摘要/Abstract

摘要： <正> 人类免疫球蛋白同种异型(human immunoglobulin allotypes)包括Gm、Km及Am等系统.在法医学实践中可用于个人识别和亲子鉴定.关于人精液中同种异型因子的检测报道极少,我们应用抗G2m(n)因子的检测国内外均未见报道,我

Abstract: This paper reports an enzyme-linked immunosorbent assay inhibition test with a monoclonal antibody(SH-2l)for G2m(n) typing of immunoglobulin allotype in human semen stains. By the ELISA-I test, either 3μl semen or 1×1 cm~2 soman stains could be typed correctly for G2m(n) factor. The results of G2m(n) typing were identical between semen and serum of 24 individuals and between semina of 112 individuals and semen stains which were derived from the semina,respectively.

Key words: Immunoglobulin allotypes G2m(n) factor Monoclonal antibody Enzyme-linked immunosorbent assay inhibition Discrimination of semen stains

侯一平;苟清;吴梅筠;. 单克隆抗体酶联免疫吸附抑制试验测定精斑G2m(n)因子[J]. 法医学杂志, 1991, 0(3): 6-9.

HOU YIPING GOU QING WU MEIYUNDEPARTMENT OF FORENSIC SEROLOGYWEST CHINA UNIVERSITY OF MEDICAL SCIENCES. G2M(N) Typing in Human Semen Stains by ELISA-I Test with Monoclonal Antibody[J]. , 1991, 0(3): 6-9.