法医学杂志

法医学杂志 ›› 1992, Vol. 0 ›› Issue (1): 26-28+4.

• 论文 • 上一篇    下一篇

小鼠死后肝细胞超微结构的扫描电镜观察

黄飞骏;廖志钢;彭雪梅;董培正;   

  1. 华西医科大学法医学系,华西医科大学法医学系,华西医科大学法医学系,华西医科大学法医学系 成都 610041,成都 610041,成都 610041,成都 610041
  • 发布日期:1992-02-25 出版日期:1992-02-28

THE POSTMORTEM ULTRASTRUCTURAL CHANGES OF THE LIVER CELL IN MICE OBSERVED BY SCANNING ELECTRON MICROSCOPY

HUANG FEIJUN SCHOOL OF FORENSIC MEDICINE WEST CHINA UNIVERSITY OF MEDICAL SCIENCES; CHENGDU 610041   

  • Online:1992-02-25 Published:1992-02-28

被引次数

1

摘要： 本研究采用冰冻断裂技术处理组织,在扫描电镜下观察实验性小鼠死后温度为20℃的潮湿环境肝细胞超微结构的改变.结果发现:死后3小时,肝细胞的内质网轻度扩张,线粒体肿胀等形态学改变,细胞核改变较晚.肝细胞超微结构的改变,与死后经过时间有关,亦可作为推断机体死后经过时间的形态学指标.作者还讨论了冰冻断裂技术和扫描电镜观察细胞超微结构的优点和局限性.

关键词: 肝细胞, 超微结构, 冰冻断裂, 扫描电镜

Abstract: Experimental postmortem ultrastructural changes of the liver cell in mice in moisty temperature at 20℃ with method of freeze-fracture were observed by scanning electron microscopy (SEM). The results showed slightly extended endoplasmic reticulum and enlarged mitochondria in mice liver cellls 3 hour after death. The neucli of liver cell did not change until 6 to 1.2 hours after death. The ultrastructural changes of liver cell were found to correlate with the post-mortem intervals. And the results also indicated that it could be used as morphological indicator to determine the time of death. The advantages and disadvantages of freeze-fracture method and resolution of SEM for obser. vation of ultrastructure were disccussed as well.