法医学杂志

法医学杂志 ›› 1992, Vol. 0 ›› Issue (2): 49-51+9.

• 论文 •    下一篇

实验性早期心肌梗死的肌红蛋白免疫组织化学研究

姚季生;姚申;应爱娣;颜召文;佘伟良;   

  1. 上海第二医科大学法医学教研室,上海第二医科大学法医学教研室,上海第二医科大学法医学教研室,上海第二医科大学法医学教研室,上海第二医科大学法医学教研室 上海200025,上海200025,上海200025,上海200025,上海200025
  • 发布日期:1992-04-25 出版日期:1992-04-28

IMMUNOHISTOCHEMICAL STUDY 0F MYOGLOBIN IN EXPERIMENTAL EA-RLY MYOCARDIAL INFARCTION

YAO JISHENG. ET AL. DEPARTMENT OF FORENSIC MEDICINE,SHANGHAI SECOND MEDICAL UNI-VERSITY, SHANGHAI.200025   

  • Online:1992-04-25 Published:1992-04-28

被引次数

10

摘要： 结扎大鼠冠状动脉前降支,制造早期(5分钟到24小时)心肌梗死模型.用抗肌红蛋白抗体免疫组织化学技术(PAP法)观察大鼠心肌梗死区肌红蛋白脱失(缺染)情况,结果表明:冠状血管结扎后30分钟,梗死区少数心肌出现轻度不完全性缺染;结扎2-6小时,梗死区心肌缺染范围扩大呈带状、条状、片状;结扎lO-24小时,梗死区心肌缺染范围达全层,大部分属完全性缺染,与非梗死区分界清晰.

关键词: 早期心肌梗死, 肌红蛋白, 免疫组织化学法

Abstract: We produced the animal model of the early myocardial infarction (5 min to 24 h) by ligating the left anterior descending coronary artery of-rats. The immunohistochemical methods (PAP) of myoglobin antibody were performed to observe the myoglobin defects of myocytes in infarcting area of rat hearts. The results demonstrated that: Slight imcomplete myoglobin defects in few myo-cytes of infarcting area were noted at 30 min after coronary ligation. At 2-6h after coronary ligation, the limits of the myoglobin defects was extended and become ribbon strip or sheet in shape. At 10- 24h after ligation, the myoglobin defects of myoc-ytes in infarcting area attain total layer of myoc-ardium, the majority of myoglobin defects was complete and could be clearly differentieted from the non-infarcting area.