法医学杂志

法医学杂志 ›› 1993, Vol. 0 ›› Issue (1): 5-8+48.

• 论文 • 上一篇    下一篇

人体组织酯酶D分型及检出时限研究

吴文庆;刘志萍;张其英;王永忠;   

  1. 上海医科大学法医学系,上海医科大学法医学系,上海医科大学法医学系,镇江医学院病理学解剖教研室 上海 200032,上海 200032,上海 200032,镇江 212001
  • 发布日期:1993-02-25 出版日期:1993-02-28

STUDY ON PHENOTYPING AND DETERMINATION LIMITS OF ED IN 14 KINDS OF ORGAN TISSUES.

WENQING LIU ZHIPING WANG YONGZHONG ZHANG QIYIN DEPARTMENT OF FARENSIC MEDICINE,SHANGHAI MEDICAL UNIVERSITY;SHANGHAI.(200032)   

  • Online:1993-02-25 Published:1993-02-28

摘要： 本文应用等电聚焦技术对12例(男7例,女5例)尸体组织 EsD 分型及检出时限进行研究。死后24—48小时的14种组织,除皮肤外均能检出 EsD 并能正确分型。组织中 EsD 检出时限37℃为3天;室温为3周;4℃为5周。

关键词: 人体组织, 酯酶 D, 等电聚焦

Abstract: Polymorphism of EsD was investigated by isoelectrical fo- cusing in 14 kinds of organ tissuse,that is,in brain,heart,liv- er,spleen,lung,kidney,pancreas,small intestine,large intes- tine,muscle,skin,ovary,uterus,and testis.The samples were obtained from 7 male and 5female cadavers who were medicolegally autopsied within 48h after death.The results showed that except skin,all the 13 kinds of organs included 5 EsD 1-1,6 EsD 2-1,and 1 EsD 2-2.The results for the determination limits of EsD types in 13 organ tissues stored at 37℃,room temperature and 4℃ showed that all the tissues examined were correctly typed for EsD at 37℃ for periods of up to 3 days,at room temperature for periods of up to 3 weeks and at 4℃ for periods of up to 5 weeks.