法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (1): 21-24+6.

• 论文 • 上一篇    下一篇

PGM_1和EAP同步等电聚焦电泳分型

柳燕   

  1. 司法部司法鉴定科学技术研究所!上海,200063
  • 发布日期:1998-02-25 出版日期:1998-02-28

Simultaneous phenotyping of EAP and PGMI by PAGIF

LIU YAN(LNSLITULE OF FORENSIC SCIENCE, MINISLRY OFJUSTICE,P. R.CHINA, SHANGHAI 200063)   

  • Online:1998-02-25 Published:1998-02-28

摘要： 采用薄层等电聚焦电泳PAGIF，结合反转显色，同时对EAP及PGM1亚型分型。结果在同一块凝胶板以两个表面分别显现出清晰的同工酶谱带，结果互不干扰。EAP检出AA、BB、BA三种表型PGM1检出十种亚型。PAGIF结合双面反转显色可同时对EAP及PGM1亚型分型，累积个体识别率为0．87，可应用于亲子生定。

关键词: 同工酶, 同步分型, 个体识别率, 遗传指标, 等电聚焦电泳

Abstract: The study is to find new genetic markers of simultaneousphenotyping and to raise the determinating power ofisoenzymes. Simultaneous electrophoresis of EAP andPGMI by thin-layer PAGIF combined with reversezymogram developing was applied and clear isoenzymebands was observed on both surface of a gel withoutinterference.The method can do typing of the EAP andPGMl at one gel and the accumulative DP is 0.87 It canbe used in paternity test.

Key words: isoenzyme, Simultaneous phenotyping, DP genetic marker, isoelectric focusing electrophesis