关键词: STR位点复合检测, 荧光标记, 微量生物学检材

Abstract: Objective Chinese Hans population study of 8 STR loci was carried out in 350 unrelated individuals. Methods the 8 STR loci(vWA,TH01,TPOX,CSF1PO,D5S818、 D13S317、 D7S820、 D16S539) and a sexual distinction locus Amelogenin, were amplified simultaneously in one PCR reaction tube, the products were separated by electrophoresis with PE－ 377 DNA sequencer, and analyzed by Genescan analysis software, then the accurate PCR fragment length and designation of genotypes were finished automatically by the sequencer. Results 63 alleles and 2 sex determination alleles were found in Hans population altogether; the minimum allele frequency in Chinese Hans population at these loci was 0.17％ , the maximum was 50.17％ ; the fragments were 119－ 327 base pairs long; the TDP of this system reached 99.999 999 98％ ; this system inherited steadily, and was accord with Mandel inheritance rule, no gene mutation was found, all loci met Hardy－ Weinberg equilibrium, but the frequencies of Hans population differed from those of African－ Americans, Caucasion－ Americans and Hispanic－ Americans. Conclusion this method is good for identification of little amount and degraded DNA samples from blood stain, semen stain mixed with vaginal excretion, hair root, cigarette root, muscle tissue and ancient bone. It can also make a foundation of establishing the National DNA Database. [

Key words: STR multiplex, population study, forensic application