法医学杂志

法医学杂志 ›› 2001, Vol. 0 ›› Issue (4): 193-195.

• 论文 •    下一篇

大鼠Fn剪接异型体分布及皮肤损伤后表达研究

陈忆九,刘宁国,黄晓华,李莉   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063 ,上海200063 ,上海200063 ,上海200063
  • 发布日期:2001-08-25 出版日期:2001-08-28

In vivo distribution and expression after skin injury of fibronectin splicing variants

CHEN YI-JIU, LIU NING-GUO, HUANG XIAO-HUA,ET AL (THE INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, SHANGHAI 200063)   

  • Online:2001-08-25 Published:2001-08-28

被引次数

23

摘要： 目的研究成年大鼠、新生幼鼠纤维连接蛋白剪接异型体EⅢA、EⅢB体内分布及皮肤切创、挫伤后表达情况,以期为法医学损伤经历时间研究探索新指标及选择阳性对照。方法组织匀浆后抽提总RNA,逆转录聚合酶链反应(RT-PCR)技术扩增目标条带。结果(1)正常皮肤EⅢA+、EⅢB+不表达,伤后18hEⅢA+位点高丰度表达,EⅢB+位点变化不明显,两种致伤方式表达结果差别不大;(2)成年大鼠各器官分布情况依次为脑、心、肝。结论EⅢA+可作为皮肤损伤研究的敏感指标。

关键词: 皮肤损伤, 纤维连接蛋白EⅢA, 可变剪接

Abstract: Objective In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EⅢA and EⅢB were studied. Methods Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique. Results (1)EⅢA+ and EⅢB+ of normal skin were not expressed, whereas EⅢA+ after 18 hours' injury was expressed abundantly. No obvious charge of EⅢB+ was detected. The differences between results of using two methods of injury were not significant.(2)The distribution of fibronectin variants in different adult organs were in the following order: brain >heart >liver. Conclusion EⅢA+ may be used as a sensitive criterion for research of skin injury.

Key words: skin injury, fibronectin, alternative splicing