法医学杂志

法医学杂志 ›› 2002, Vol. 0 ›› Issue (1): 12-14+6.

• 论文 • 上一篇    下一篇

大鼠早期心肌缺血心肌肌钙蛋白T脱失的免疫组化研究

邓大中,陈玉川,胡丙杰,蔡明屈   

  1. 广东高等公安专科学校技术系,中山医科大学法医学系,中山医科大学法医学系,广东高等公安专科学校技术系 广东广州510232 ,广东广州510089 ,广东广州510089 ,广东广州510232
  • 发布日期:2002-02-25 出版日期:2002-02-28

Immunohistochemical study with depletion of cardiac troponin T in rats on early myocardial ischaemia

DENG DA-ZHONG1,CHEN YU-CHUAN2,HU BIN-JIE2,ET AL. (1.DEPARTMENT OF CRIMINAL TECHNIQUE,GUANGDONG POLICE COLLEGE, GUANGZHOU 510232; 2.DEPARTMENT OF FORENSIC PATHOLOGY, SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES, GUANGZHOU 510089)   

  • Online:2002-02-25 Published:2002-02-28

被引次数

45

摘要： 目的研究早期心肌缺血大鼠模型上心肌肌钙蛋白T的脱失情况。方法应用免疫组化LSAB法显色观察。结果缺血15分钟,大鼠心肌肌钙蛋白T即有明显脱失,随缺血时间的延长,脱失面积呈扩大趋势。计算机图像分析显示缺血大鼠心肌肌钙蛋白T的脱失面积与正常大鼠心肌之间存在显著性差异。结论心肌肌钙蛋白T是诊断早期心肌缺血的较为敏感的指标之一。

关键词: 早期心肌缺血, 心肌肌钙蛋白T, 免疫组化技术, 计算机图像分析

Abstract: Objective To study the depletion of cardiac troponin T in Rats of early myocardial ischaemia. Methods Immunohistochemical method (LSAB) was used and the depletion area of CTnT in myocardial ischaemia were measured by the computer image analysis. Results There is obviously depletion of CTnT after 15 min in myocardial ischaemia of rats, the mean of CTnT depletion of myocardial ischaemic group is significant difference compared with that of the control group(P< 0.01), and the depletion area increased with the prolongagtion of ischaemic time. Conclusion The depletion of CTnT is one of sensitive signs of early myocardial ischaemia.

Key words: early myocardial ischaemia, cardiac troponin T, immunohistochemistry, image analysis system