OTS-2.2S基因芯片对人脑挫伤后基因表达差异的初步研究

法医学杂志 ›› 2004, Vol. 0 ›› Issue (2): 77-80+1.

OTS-2.2S基因芯片对人脑挫伤后基因表达差异的初步研究

周亦武,张益鹄,刘艳,邓伟年,王成毅

华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系湖北武汉430030,汕头大学医学院法医学教研室,广东汕头515031 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030

发布日期:2004-04-25 出版日期:2004-04-28

A primary study on the gene expression profiling of human brain contusion by cDNA microarray

ZHOU YI-WU, ZHANG YI-GU, LIU YAN, ET AL. ( FACULTY OF FORENSIC MEDICINE,TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN 430030,CHINA)

Online:2004-04-25 Published:2004-04-28

摘要/Abstract

摘要： 目的利用基因芯片技术,研究人挫伤脑组织中基因表达差异。方法从脑组织提取mRNA,通过OTS-2.2S基因芯片,研究脑挫伤组织与对照脑组织细胞原癌及抑癌相关基因特异性表达差异。结果发现在挫伤脑组织中,HoJ-1和KIAAOO65基因表达水平显著下降,而p107mRNA表达水平显著升高。结论在脑挫伤中仅发现3条基因的表达有显著性差异;本研究是对脑损伤后基因表达水平及其法医学意义进行的探索性研究。

关键词: 微矩阵基因芯片, 基因表达谱, 脑挫伤, 原癌基因, 抑癌基因

Abstract: Objective To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray. Mathods The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues. Results Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1(HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue. Conclusion The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.

Key words: cDNA microarray, gene expression profiling, brain contusion, oncogene, tumors suppressed gene

周亦武;张益鹄;刘艳;邓伟年;王成毅. OTS-2.2S基因芯片对人脑挫伤后基因表达差异的初步研究[J]. 法医学杂志, 2004, 0(2): 77-80+1.

ZHOU YI-WU;ZHANG YI-GU;LIU YAN;ET AL. ( FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA). A primary study on the gene expression profiling of human brain contusion by cDNA microarray[J]. , 2004, 0(2): 77-80+1.

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