法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 117-119.

• 论文 • 上一篇    下一篇

SSCP法mtDNA分型的法医学应用

艾红伟;余纯应;赵贵森;黄代新;柯技;岳霞;杨庆恩;   

  1. 华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系 湖北武汉430030,湖北武汉430030,湖北武汉430030,湖北武汉430030,湖北武汉430030,湖北武汉430030,湖北武汉430030
  • 发布日期:2006-04-25 出版日期:2006-04-28

The Application of PCR-SSCP in Forensic mtDNA Typing

AI HONG-WEI, YU CHUN-YING, ZHAO GUI-SEN, HUANG DAI-XIN, KE JI, YUE XIA, YANG QING-EN (DEPARTMENT OF FORENSIC MEDICINE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY, WUHAN 430030,CHINA)   

  • Online:2006-04-25 Published:2006-04-28

摘要： 目的探讨SSCP法mtDNA分型在法医鉴定实践中的意义。方法PCR扩增mtDNAHV-I和HV-II的序列多态性片段,直接用SSCP技术分型。对70个湖北汉族真三联家系和140例随机个体进行检测,比较家系中mtDNA的单倍型SSCP图谱。统计分析SSCP法用于两个高变区在母系确定、个人识别中的意义和鉴别能力。结果在70个家系中,母亲和孩子的HV-I、HV-IISSCP带型完全相同;家系中父亲与母子的HV-I图谱不同占98.57%;HV-IISSCP图谱不同占97.13%。140例随机个体的HVI、HVII区分别检出21、16种单倍型,GD值分别为0.9556、0.9356。结论mtDNA-SSCP分型在嫌疑人筛查和母系亲缘关系推断中有实际应用价值。

关键词: 线粒体DNA, 单链构象多态性, 个人识别, 母系家系

Abstract: Objective To study the application of PCR-SSCP in forensic mtDNA typing. Methods Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed. Results In 70 family trios,the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD=0.9556; 16 haplotypes were found in HVII, GD=0.9356. Conclusion PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.

Key words: mtDNA, single strand conformation polymorphisms, individual identification, maternal genealogy