法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (3): 191-193.DOI: 10.3969/j.issn.1004-5619.2014.03.009

• 技术与应用 • 上一篇    下一篇

PowerPlex®21与GoldeneyeTM 20A试剂盒的一致性验证及法医学应用

任  贺1，刘  莹2，张庆霞2，焦章平2   

  1. （1. 北京警察学院，北京 102202； 2. 北京市公安局司法鉴定中心，北京 100192）
  • 发布日期:2014-06-25 出版日期:2014-06-28
  • 通讯作者: 刘莹，女，副主任法医师，硕士，主要从事法医物证学研究；E-mail：snow.ly@163.com
  • 作者简介:任贺（1978—），女，北京人，主检法医师，硕士，主要从事法医物证学教学与研究；E-mail：snoopy_fr@sohu.com

Consistency Study of PowerPlex®21 Kit and GoldeneyeTM 20A Kit and Forensic Application

REN HE1, LIU YING2, ZHANG QING-XIA2, JIAO ZHANG-PING2   

  1. （1. Beijing Police College, Beijing 102202, China; 2. Forensic Medical Examination Center of Beijing Public Security Bureau, Beijing 100192, China）
  • Online:2014-06-25 Published:2014-06-28

摘要： 目的 确认PowerPlex®21试剂盒与GoldeneyeTM 20A试剂盒分型结果的一致性。方法 应用两试剂盒对205名北京汉族无关个体血样DNA进行复合扩增，观察19个重叠STR基因座分型的一致性，并统计D1S1656的遗传多态性。 结果 所有19个重叠基因座分型相同，两个试剂盒的杂合基因座峰高比例差异无统计学意义（P>0.05）。D1S1656杂合度为0.878，个人识别率为0.949，三联体非父排除率为0.751，二联体非父排除率为0.506，多态信息含量为0.810。 结论 PowerPlex®21试剂盒与GoldeneyeTM 20A试剂盒分型结果一致性好，引物设计合理；D1S1656多态性好，可用于人类遗传分析及法医学中的亲子鉴定和个人识别。

关键词: 法医遗传学；多态现象, 遗传；短串联重复序列；D1S1656；一致性验证；北京；汉族

Abstract: Objective To ensure the consistency of genotype results for PowerPlex®21 kit and GoldeneyeTM 20A kit. Methods The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained. Results All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference （P>0.05）. The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymorphism information content was 0.810. Conclusion PowerPlex®21 kit and GoldeneyeTM 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.

Key words: forensic genetics, polymorphism, genetic, STR, D1S1656, consistency verifying, Beijing, Han nationality

中图分类号: