法医学杂志

法医学杂志 ›› 1991, Vol. 0 ›› Issue (4): 169-172+.

• 论文 • 上一篇    下一篇

用M13噬菌体作探针进行人的DNA限制性片段长度多态性分析

张小为;蓝翎;段秉章;霍振义;黄英;   

  1. 北京市刑事科学技术研究所,北京市刑事科学技术研究所,北京市刑事科学技术研究所,北京市刑事科学技术研究所,北京市刑事科学技术研究所 北京 100007,北京 100007,北京 100007,北京 100007,北京 100007
  • 发布日期:1991-08-25 出版日期:1991-08-28

THE ANALYSIS OF DNA RFLPs USING M13 BACTERIOPHAGE AS A PROB

ZHANG XIAOWEI, ET AL, BEIJING FORENSIC SCIENCE INSTITUTA BAIJING. (100007)   

  • Online:1991-08-25 Published:1991-08-28

被引次数

11

摘要： 用M13噬菌体作为探针,可以检测人和动物基因组DNA中高度可变的小卫星区域,其有效顺序是该噬菌体基因Ⅲ中的两组15bp的串联重复。本文介绍的是采用随机引物与PCR过程相结合的方法标记M13噬菌体,得到高比活性的探针,与经HinfⅠ消化的人DNA杂交,产生具有高度多态性的DNA图谱,并与33.15探针杂交的DNA图谱进行了比较,证明M13噬菌体是进行DNA多态性分析的一种非常有用的探针。

关键词: M13噬菌体, 小卫星DNA, 限制性片段长度多态性(RFLPs)

Abstract: The hypervarieble minisatellite regions in human, animal and plant genome DNA can be detected by M13 bacteriophage as a probe.Its effective sequence was traced to two clusters of 15-base pair tandem repeats within the gene 3 of the bacteriophage. In this paper we utilized a unipue method of PCR to label M13 phage DNA and yielded a high specific activity. Human DNA digested with Hinfl was hybridized with labelled M13 and 33.15 probes. Highly polymorphic DNA fingerpriuts were obtained witb both of them. A practical casework was tested. (Original article on page 169)