法医学杂志

法医学杂志 ›› 1994, Vol. 0 ›› Issue (3): 0-0.

• 论文 •    

RP—HPLC测定全血中三环类抗抑郁药的研究

李双庆，刘耀，何毅，高利生，周恒智   

  1. 北京公安部第二研究所
  • 发布日期:1994-06-25 出版日期:1994-06-28

RP-HPLC SIMULTANEOUS DETERMINATION OF TRICYLIC ANTIDEPRESSANTS IN HUMAN WHOLE BLOOD

LI SHANGQING;LIU YAO; HEYI;GAO LISHINGG;ZHOU HENGZHI(INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF PUBLIC SECURITY;BEIJING,100038)   

  • Online:1994-06-25 Published:1994-06-28

摘要： 本文建立了反相高效液相色谱法，测定全血中三环类抗抑郁药：阿米替林、多虑平、三甲丙咪嗪、氯丙咪嗪、丙咪嗪、去甲丙咪嗪。以乙酸苯胺为内标物。血样碱化后，用正己烷：异戊醇（９９∶１）提取，０．１ｍｏｌ／Ｌ硫酸及提。色谱柱为ＨｙｐｅｒｓｉｌＣｌｓ；流动相为乙腈：０．０１ｍｏｌ／Ｌ磷酸二氢钾（含０．０１ｍｏｌ／Ｌ二乙胺的水溶液并加磷酸调至ｐＨ３．０（３∶７）；２１０ｎｍ检测。线性范围为０．０１５６—２．０ｕｇ／ｍｌ，最低检出浓度为１０ｎｇ／ｍｌ（在全血中）。本法简便、快速，适用于法庭毒物分析及临床治疗药物监测。

关键词: 反相液相色谱法, 全血, 三环类抗抑郁药的测定

Abstract: A reversed-phase HPLC method for simultaneous determination of amitriptyline,doxepin,trimipramine,clomipramine,inipramine and their major metabolites despramine in human whole blood was described. The method involved the use of acetanilideas and as internal standard. Blood samples were extracted at basic pH with he-cane: isoamyl(99∶1,V/V) followed by back extraction into 0.1 mol/L sulfuric acid, Thechromatographic separation was accomplished with amobile phase comprising acetonitrile - aqueous solution containing 0.01mol/L potassium dihydrogenphosphate and 0. 01 mol/L diethlamine with phosphoric acid adjusted to pH 3. 0 (2∶8,V/V).The mobile phase was pumped at flow rate 2. 0 ml/min.The column used Hypersil C18. Detection isachieved at 210nm, the lower limit of sensitivity was 10ng/ml. The recoveries of tricyclic antidepressants was 58.4 - 107. 6%'the coefficients of variation to within-daywas 1. 78-10. 07% and day to day was 3. 01 -11.05 %.This method is suitable in forensic toxicology analysis and TDM for tricyclic antidepressants.

Key words: RP-HPLC method, whole blood, determination of the tricylic antidepressant