法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (1): 11-14，20.DOI: 10.12116/j.issn.1004-5619.2019.491115

• 论 著 • 上一篇    下一篇

死后血浆和溶血样本中IgE的稳定性

王天琦1 ，王天方2 ，贾宇晴1 ，曹志鹏1 ，朱宝利1   

  1. 1. 中国医科大学法医学院，辽宁 沈阳 110122；2. 天津市公安局河西分局物证鉴定所，天津 300061
  • 收稿日期:2019-11-23 发布日期:2021-02-25 出版日期:2021-02-28
  • 通讯作者: 朱宝利，男，教授，博士研究生导师，主要从事法医病理学和法医病理生理学教学、科研及鉴定；E-mail：zhu1127@hotmail.com
  • 作者简介:王天琦（1994—），男，硕士研究生，主要从事法医病理学和法医病理生理学研究；E-mail：1252527890@qq.com
  • 基金资助:
    国家自然科学基金资助项目（81273343）；辽宁省科技厅自然科学基金面上项目（20180530004）

Stability of IgE in Postmortem Plasma and Hemolyzed Samples

WANG Tian-qi1 , WANG Tian-fang2 , JIA Yu-qing1 , CAO Zhi-peng1 , ZHU Bao-li1   

  1. 1. School of Forensic Medicine, China Medical University, Shenyang 110122, China; 2. Institute of Foren? sic Science, Hexi Branch of Tianjin Public Security Bureau, Tianjin 300061, China
  • Received:2019-11-23 Online:2021-02-25 Published:2021-02-28

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摘要： 目的 探究死后血浆和溶血样本中免疫球蛋白E（immunoglobulin E，IgE）经过不同保存条件及冻 融处理后的稳定性。 方法 选取39例死后48 h内非冷冻尸体的心血样本，其中20例取血浆样本，19例取 全血制成溶血样本。将样本置于-20 ℃、4 ℃、25 ℃条件下保存28 d及-80 ℃条件下保存1年以探究IgE在不 同保存条件下的稳定性。利用5次反复冻融处理样本探究死后血浆及溶血样本IgE的冻融稳定性。应用 电化学发光法检测处理前后血浆和溶血样本中 IgE 的浓度。 结果 血浆样本中 IgE 在-20 ℃、4 ℃、25 ℃ 3种保存条件下的降解率接近，28 d后降解率均值在15%左右，相同条件下溶血样本中IgE降解速度快于血 浆（P<0.05），并且在25 ℃条件下降解率高于另外两种（P<0.05）。血浆样本于-80 ℃冷冻保存1年后的浓度 与冷冻前相比差异无统计学意义（P>0.05），而溶血样本在-80 ℃冷冻保存 1 年后浓度有所降低（P<0.05）。 死后血浆和溶血样本在反复冻融5次后检测结果与冻融前相比差异无统计学意义（P>0.05）。 结论 死后 血浆及溶血样本中IgE具有良好的冻融稳定性。IgE在死后血浆样本中的稳定性优于溶血样本，因此在实 际案例中建议及早分离血浆保存待测。

关键词: 法医病理学, 血浆, 全血, 免疫球蛋白E, 稳定性, 溶血

Abstract: Objective To investigate the stability of IgE in postmortem plasma and hemolyzed samples under different storage conditions and freezing-thawing. Methods Thirty nine cardiac blood samples were collected from non-frozen corpses with the postmortem interval of less than 48 hours, including 20 plasma samples and 19 hemolyzed samples taken from whole blood. The samples were stored at -20 ℃, 4 ℃ and 25 ℃ for 28 d and at -80 ℃ for 1 year to evaluate the stability of IgE under different storage conditions. Repeated freezing-thawing treatment was conducted for 5 times to explore the stability of IgE in postmortem plasma and hemolyzed samples. IgE concentration in plasma and hemolyzed samples was detected by electroluminescence before and after treatment. Results The degradation rates of IgE in plasma samples under the three storage conditions, -20 ℃, 4 ℃ and 25 ℃ were close. After 28 d, the mean value was about 15%, the degradation speed of IgE in hemolyzed samples was faster than that of plasma under the same condition （P<0.05） and the degradation rate was faster than other two conditions under 25 ℃ （P<0.05）. The differences in the concentration of plasma samples after freezing at -80 ℃ for 1 year and that before freezing had no statistical significance （P>0.05）, while the concentration of hemolyzed samples was degraded after freezing at -80 ℃ for 1 year（P<0.05）. The differences between the detection results of plasma and hemolyzed samples after repeated freezing-thawing for 5 times and that before freezing-thawing showed no statistical significance （P>0.05）. Conclusion IgE has good freezing-thawing stability in postmortem plasma and hemolyzed samples. Stability of IgE is better in postmortem plasma samples than hemolyzed samples, thus it is recommended to separate plasma from postmortem blood samples as soon as possible in forensic practice.

Key words: forensic pathology, plasma, whole blood, immunoglobulin E, stability, hemolysis

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