法医学杂志

法医学杂志 ›› 1995, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    下一篇

银环蛇中毒死亡鉴定方法的实验研究──活化长碳臂生物素:夹心ABC—ELISA检测银环蛇毒

闫祖康，祝家镇   

  1. 上海医科大学法医学系，中山医科大学法医学系
  • 发布日期:1995-04-25 出版日期:1995-04-28

Identification of Death Caused by Snake Biting-Establishment of ABC-ELISA for the Detection of Krait Venom

YAN ZUKANG(FACLUTY OF FORENSIC MEDICINE, SHANGHAI MEDICALUNIVERSITY; SHANGHAI 200032), ZHU JIAZHEN (FACULTY OF FORENSIC MEDICINE, SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES:GUANGZHOU510089)   

  • Online:1995-04-25 Published:1995-04-28

摘要： 用自制活化长碳臂生物素标记马抗银环蛇毒抗体进行夹心ＡＢＣ—ＥＬＩＳＡ测试，所获参数为：最小检出量０．２５ｎｇ／ｍｌ；标准曲线最适检测范围０．２５～２５０ｎｇ／ｍｌ；批内变异＜８％，批间变异＜１６％，不同浓度回收率为７８～８９％；与同科眼镜蛇毒无交叉反应。作者从法医学鉴定的实际需要出发，利用正交设计研究了各种因素对检测的影响，结果示：蛇咬死亡动物血液和肾脏为最佳检材，最长检出时间可达３０天。本方法常规应用可靠性强。

关键词: 蛇咬伤, 蛇毒, 酶联免疫吸附测定, 法医学鉴定

Abstract: A double antibody ABC- ELISA using NHBH conjugatehas ben investigated on krait venom standards, which revealed that the optimal reaction over the range was 0.25- 250 ng/ml, intra- and inter- assay variations were lessthan 8% and 16%, recovery was 78% -89% within thesame range, the cross Teaction with cobra venom was negligible. The studies of the factors affectins detection of the venom through three orthogonal tables show that the serumand kidney tissue from the experimental animals were the most satisfaCtory samples for identifying snake biting within 720 hours after death. The results of the experiment data indicate a strong reliability for routine test.

Key words: snake-bite, venom, ELISA