法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (4): 0-0.

• 论文 •    

大麻的体内代谢物THC-COOH的分析

向平，沈敏，沈保华   

  1. 司法部司法鉴定科学技术研究所
  • 发布日期:1996-08-25 出版日期:1996-08-28

Analysis of the Main Cannabis Metabolite THC-COOH

XIANG PING;SHEN MIN AND SHEN BAOHUA (INSTITUTE OF FORENSIC SCIENSES,SHANGHAI,200061)   

  • Online:1996-08-25 Published:1996-08-28

摘要： 本文介绍了定性定量分析大麻的主要代谢物ＴＨＣ－ＣＯＯＨ的两种方法。所有试管硅烷化，尿样碱性水解。ＴＬＣ法快速、简便，适用于多份检材的同时定性分析，最低检测限为５０ｎｇ．采用衍生化、ＳＩＭ的ＧＣ／ＭＳ方法灵敏，选择性、专一性强．五氟丙酸酐衍生化方法线性范围为２０－２５０ｎｇ／ｍｌ，最低检测限１０ｎｇ／ｍｌ，变异系数为８．４％，提取回收率为５８．９％；甲基化方法线性范围１０～１００ｎｇ／ｍｌ，最低检测限５ｎｇ／ｍｌ，变异系数为５．６％，提取回收率为５３．２％．

关键词: 薄层色谱, 气－质联用仪, △~9－四氢大麻酸, 硅烷化, 衍生化

Abstract: A procedure of detection and quantitation of THC-COOH,a major metabolite of THC in urine. has been described.Prior to extraction,the tubes must be silanized.The hydrolysis of urine is necessary.Derivatizing and selected ion monitoring are oboed out to increase the sensitivity of detection.TLC is rapid for a large number of sample analysises.The method of GC/MS is sensitive.selective and specific.

Key words: TLC, GC/MS, THC-COOH, silanize, derivatize