法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (4): 0-0.

• 论文 •    

大白鼠脑干损伤后脑组织中GFAP免疫组化改变的初步研究

邓平，徐小虎，祝家镇   

  1. 中山医科大学法医学系
  • 发布日期:1996-08-25 出版日期:1996-08-28

Preliminary Report on Immunohistochcmical Changes of GFAP in Different Areas in Rat's Brain Following Brain-stem Injury

DENG PING; XU XIAOHU AND ZHU JIAZHEN(FACULTY OF PARENSIC PATHOLOGY,SUN YAT-SEN UNIVERSITY OF MEDICALSCIENCES,GUANGZHOU 510089)   

  • Online:1996-08-25 Published:1996-08-28

摘要： 采取针刺法造成实验大白鼠脑干损伤，用免疫组织化学ＬＳＡＢ法检测大脑、中脑、桥脑及延髓等不同部位脑组织中胶质原纤维酸性蛋白（ＧＦＡＰ）的改变。结果发现，生前损伤３０ｍｉｎ，大脑顶部灰质及中脑腹例中央部的ＧＦＡＰ阳性星形胶质细胞数目增多；生前损伤６０ｍｉｎ，除大脑顶部灰质及中脑腹侧中央部外．大脑脑室角周边部、桥脑背侧中央部、延髓腹侧及背侧中央部的ＧＦＡＰ阳性星形胶质细胞数目亦增多。而死后损伤者，ＧＦＡＰ阳性星形胶质细胞数目不增多。说明ＧＦＡＰ阳性星形胶质细胞数目的改变可区别脑组织的生前损伤和死后改变，并可作为脑干损伤早期的诊断指标。

关键词: 脑干损伤, GFAP, 生前损伤, 死后损伤

Abstract: Following brain stem injury,the number of GFAP-positive astrocytes in cerebral cortex and pervent-ricular area,dorsomedial and ventro-medial pafts of midbrain at 30 min antemortem,and in periventricular area,dorsomedial part of pens,dorso-medial and ventro-medial part of medulla oblongata at 60 min antemortem.Bac there is no increase of GFAP-positive astrocytes in postmortem injured brain.It indicated that the changes in number of GFAP-positive astrocytes can be used to diagnose early brain-stem injuries.

Key words: brain-stem injury, GFA-P, antermortem injury, postmortem injury