法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (4): 0-0.

• 论文 •    

降解DNA的PCR分型-电洗脱回收大片断DNA法

倪星群，郭景元，陈丽娴，郭云荣   

  1. 中山医科大学法医物证学教研室
  • 发布日期:1996-08-25 出版日期:1996-08-28

DNA Profiling from Degrative DNA by Polymerase Chain Reaction(PCR):Recovered the Larger DNA Fragments by Electrophoretic Elution

NI XINGQUN; GUO JINYUAN; CHEN LIXIAN AND GUO YUNRONG(DEPARTMENT OF FORENSIC SEROLOGY,SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES GUANGZHOU 510089)   

  • Online:1996-08-25 Published:1996-08-28

被引次数

4

摘要： 本文分析了降解ＤＮＡ作ＰＣＲ分型易失败的原因，提出了因小片断ＤＮＡ过多，阻碍引物与膜机的复性及阻断引物延伸的新现点。将降解的ＤＮＡ电泳，切除小片断ＤＮＡ，电洗脱回收较大片断ＤＮＡ，成功地对降解ＤＮＡ进行了ＰＣＲ分型．本方法简便、快速、有效。

关键词: 降解DNA, 电洗脱, 聚合酶链反应

Abstract: The cause profiling unsuccessfully the degradative DNA by the polymerase chain reaction(PCR) was analysed.A new view was advanced.There were a lot of small DNA fragments in the degradative DNA It blocked the primer annealing and the primer extension.In this article the degradative DNA was separated by agarose gel electrophoresis.The small DNA fragments were cut off The larger DNA fragments were recovered froom the gel by electrophoretic elution and were successfully typed by PCR.This method was easy to operate.

Key words: degradativc DNA, electrophoretic elution, polymerase chain reaction