法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (3): 138-140+.

• 论文 • 上一篇    下一篇

HLA-DQα和PM位点扩增分型技术在法医物证学上的应用

林源,阙庭志,李莉,赵珍敏   

  1. 司法部司法鉴定科学技术研究所!上海,200063,司法部司法鉴定科学技术研究所!上海,200063,司法部司法鉴定科学技术研究所!上海,200063,司法部司法鉴定科学技术研究所!上海,200063
  • 发布日期:1998-06-25 出版日期:1998-06-28

PCR-based Detection of had HLA-DQαand Polymarker Loci And Their Application in Forensic Science

LIN YUAN ，QUE TINGZHI， LILI，ZHAO ZHENMIN （INSTI-TUTE OF FORENSIC SCIENCES， MINISTRY OF JUSTICE ， SHANGHAI，200063）   

  • Online:1998-06-25 Published:1998-06-28

被引次数

8

摘要： 目的：对HLA－DQα和PM位点进行多态性研究并应用于实际检案。方法：用PCR结合反相杂交技术进行扩增并分型。结果：获得HLA－DQα和PM位点的基因频率分布数据。结论：上述位点具有高度多态性，累积个体鉴别能力（DP值）达99．95％，是法医学亲权鉴定和个体识别领域非常有价值的遗传标记系统。

关键词: PCR复合扩增, 反相杂交技术, 基因位点, HLA－DQα, PM

Abstract: Studies were conducted to evaluate the forensic ap-plicability of multiplex amplification of HLA -DQα and PM loci. These loci could be simultaaneously typed by re-verse dot blot approach where allele specificoligonucleoti-de probes were immobilized on a nylon membrane strip.Allele and genotype frequencies for HLA -DQα and PMloci were determined in Chinese Han population . The fre- quency data can be used in individual identification andp paternity test.

Key words: ：PCR Multiplex Amplification, Reverse Dot Blot, Gene Loci, HLA -DQα, LDLR, GYPA, HBGG, D7S8, GC