法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (3): 135-137+.

• 论文 • 上一篇    下一篇

热休克蛋白70对大鼠脑干损伤诊断的免疫组织化学研究

赵连旭,徐小虎,祝家镇,陈建国   

  1. 中山医科大学法医系!广东广州,510089,汕头大学医学院法医研究所!广东汕头,515030,中山医科大学法医系!广东广州,510089,中山医科大学法医系!广东广州,510089
  • 发布日期:1998-06-25 出版日期:1998-06-28

Immunohistochemical research on brain stem injury：expression of heat shock protein 70 in rat brain after traumatic brain stem injury

ZHAO LIANXU， XU XIAOHU， ZHU JIAZHEN， CHEN JIANGUO（FACULTY OF FORENSIC MEDICINE， SUN YAT- SEN UNIVERSITYOF MEDICAL SCIENCES， GUANGZHOU 510089）   

  • Online:1998-06-25 Published:1998-06-28

被引次数

53

摘要： 为了解脑干在受到创伤后作为降解异常蛋白的分子伴侣（molecularchaperones）之一的热休克蛋白70（heatshockprotein70HSP70）在中枢神经系统的表达情况，用免疫组化法对Wistar大鼠脑干针刺损伤模型的HSP70在损伤前及损伤后1、3、6、12、24小时的中枢神经各区的表达数量、分布变化进行检测。在针刺后1小时脑组织HE染色尚无明显特异性改变的情况下，大脑及小脑内HSP7免疫活性阳性的血管内皮细胞、神经胶质细胞显著增加，在损伤灶局部周围出现HSP7I免疫活性阳性神经元细胞增多，伤后3小时该处HSP7阳性神经元数量显著多于伤前及同一时间点的其它处脑组织，伤后24小时HSP7阳性细胞依然广泛存在。脑干的外伤性损伤可导致脑组织的蛋白质结构发生改变，脑干局部HSP70阳性神经元数量增多可作为脑干损伤定位诊断的依据。

关键词: 热休克, 蛋白脑, 干损伤, 免疫组织化学

Abstract: The aim of this study was to determine the expres-sion of heat shock protein 70 （HSP70）as molecular chap-erones ，following tratunatic brain stem injury （TBSI） Theexpression HSP70 was detected in respective brain re-gions of rats 1h, 3h, 6h, 12h, 24h after brain stem stab-bing wound. Numerous HSP70 immunoreactivity positive blood vessel endothelioid and glia cells were found in ce-rebral, cerebellar cortex and hippocampus after lh, withH. E stain unchanged. In the area near the focal of brainstem injury ，HSP70 immunoreactivity positive neurons in-creased significantly after 1h of injury, reaching the high-est level ds 3h, much higher than in other areas.HSP70 immunoreactivity positive cells still existed after24h of injury. It was suggested that TBSI cause the de-fectiveness of protein structure in brain， and induceHSP70 proteolysis of denature Protein including mistold-ing or aggregate. TBSI can be diagnosed regionally by theincreasing of HSP70 immunoreactivity positive neurons in the focal of brain stems.

Key words: heat shock proteins, traumatic brain stem injury, immunohistochemistry