法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (6): 409-412.DOI: 10.3969/j.issn.1004-5619.2011.06.003

• 论著 • 上一篇    下一篇

大鼠局灶性脑挫伤后Homer蛋白表达变化

王福远1，2，李如波1，梁红霞1，沈  宇3，郭晓冲1，王正印1   

  1. （1. 中国医科大学 法医学院法医病理学教研室，辽宁 沈阳 110001； 2. 宁波市公安局 鄞州分局，浙江 宁波 315100； 3. 苍南县公安局 刑事技术大队，浙江 温州 325800）
  • 发布日期:2011-12-25 出版日期:2011-12-28
  • 通讯作者: 通信作者：李如波，男，博士，教授，主要从事脑死亡及弥漫性脑损伤的法医病理学研究；E-mail：rbli@mail.cmu.edu.cn
  • 作者简介:王福远（1983—），男，辽宁普兰店人，硕士研究生，主要从事颅脑损伤研究；E-mail：wfy021021@163.com
  • 基金资助:

    辽宁省高等学校科研项目（2008828）

Homer Protein Expression after Focal Brain Contusion in Rats

WANG FU-YUAN1,2, LI RU-BO1, LIANG HONG-XIA1, SHEN YU3, GUO XIAO-CHONG1, WANG ZHENG-YIN1   

  1. （1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China; 2. Yinzhou Branch of Ningbo Public Security Bureau, Ningbo 315100, China; 3. Criminal Technology Branch, Cangnan Public Security Bureau, Wenzhou 325800, China）
  • Online:2011-12-25 Published:2011-12-28

被引次数

11

摘要： 目的 观察局灶性脑挫伤后Homer蛋白表达的变化规律，探讨其表达变化与损伤时间的相关性。方法 建立大鼠钝力性局灶性脑挫伤模型，应用免疫组织化学技术和Western印迹法观察脑损伤后不同时间脑组织中Homer蛋白表达的变化。 结果 对照组、假手术组及伤后0.5 h组脑组织中有少量Homer蛋白染色阳性细胞，伤后3 h组脑组织Homer蛋白染色阳性细胞增多，伤后12 h组阳性细胞数达到高峰，伤后1 d起阳性细胞数持续下降，至伤后7 d降到基础水平。Homer蛋白染色及Western印迹法显示，Homer蛋白表达量在相邻组间差异均具有统计学意义。 结论 大鼠钝力性局灶性脑挫伤后损伤周边区Homer蛋白表达变化与脑挫伤时间具有相关性。

关键词: 法医病理学, 脑损伤, 印迹法,蛋白质, 免疫组织化学, Homer蛋白, 大鼠

Abstract: Objective To investigate Homer protein expression after focal brain contusion and explore the relationship between expression and injury time. Methods Focal brain contusion in rats was established and Homer protein expression in brain at different injury intervals after contusion was detected by immunohistochemistry and Western blotting. Results A small amount of Homer positive expression cells were detected in control group, sham operated group and experimental group （0.5 h after contusion）. The amount of Homer positive expression cells increased after 3 h and reached peak 12 h after contusion. The amount of positive cells continued to decrease 1 d after contusion and to the base level 7 d after contusion. Homer protein expression based on immunohistochemistry and Western blotting had statistical difference among adjacent groups. Conclusion Expression of Homer protein near the focal contusion area shows time dependence after brain contusion in rats.

Key words: forensic pathology, brain injuries, blotting, Western, immunohistochemistry, Homer protein, rats

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