法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (6): 405-408,412.DOI: 10.3969/j.issn.1004-5619.2011.06.002

• 论著 • 上一篇    下一篇

氯胺酮对PC12细胞增殖及凋亡的影响

左媛宜1，2，3，赵彦伯4，蒋小岗3，顾振纶3，郭次仪3，卞士中1，2   

  1. （1. 苏州大学 医学部法医学系，江苏 苏州 215123； 2. 苏州大学 司法鉴定所，江苏 苏州 215000； 3. 苏州中药研究所，江苏 苏州 215006； 4. 昆山市公安局 刑警大队法医室，江苏 昆山 215300）
  • 发布日期:2011-12-25 出版日期:2011-12-28
  • 通讯作者: 通信作者：卞士中，男，主任法医师，主要从事法医毒理学和法医病理学研究；E-mail：bianshizhong@ suda.edu.cn 蒋小岗，男，副教授，主要从事分子药理学研究；E-mail：jiangxiaogang@suda.edu.cn
  • 作者简介:左媛宜（1988—），女，江苏盐城人，硕士研究生，主要从事法医毒理学和法医病理学研究；E-mail：zuoyuanyi060@163.com

Effects of Ketamine on Proliferation and Apoptosis of Pheochromocytoma Cell

ZUO YUAN-YI1,2,3, ZHAO YAN-BO4, JIANG XIAO-GANG3, GU ZHEN-LUN3, GUO CI-YI3, BIAN SHI-ZHONG1,2   

  1. （1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 2. Forensic Judicial Appraisal Institute, Soochow University, Suzhou 215000, China; 3. Suzhou Institute of Chinese Materia Medica, Suzhou 215006, China; 4. Department of Forensic Medicine, Criminal Police Brigade of Kunshan Public Security Bureau, Kunshan 215300, China）
  • Online:2011-12-25 Published:2011-12-28

摘要： 目的 探讨氯胺酮对肾上腺嗜铬细胞瘤（pheochromocytoma，PC12）细胞的增殖抑制和诱导凋亡的作用及其机制。 方法 以大鼠PC12细胞为多巴胺能神经元的细胞模型，分别以0.9、1.2、1.5、1.8、2.1 mmol/L浓度的氯胺酮加入培养的PC12细胞中，分别培养12、24、48、72 h后，用MTT实验检测细胞的活力，Hoechst染色观察其凋亡的形态，用流式细胞仪检测不同浓度的氯胺酮加入PC12细胞培养48 h后细胞凋亡率以及用Western印迹法检测bax、bcl-2的蛋白表达。 结果 加入不同浓度的氯胺酮，随着培养时间的延长，PC12细胞的活力显著下降，Hoechst染色可观察到明显凋亡现象，流式细胞仪检测显示随着氯胺酮浓度的增加，细胞凋亡率显著增加。 结论 氯胺酮可抑制PC12细胞增殖，并可显著诱导PC12细胞凋亡，且呈浓度依赖性，其作用机制可能与促进细胞内bax以及抑制bcl-2的表达有关。

关键词: 法医病理学, 氯胺酮, PC12细胞, 细胞增殖, 细胞凋亡, 大鼠

Abstract: Objective To explore the effect of ketamine on adrenal pheochromocytoma（PC12） cell proliferation inhibition and induction of apoptosis and its mechanism. Methods PC12 cells of rats were models for dopaminergic neuron. PC12 cells were cultured with ketamine at concentrations of 0.9, 1.2, 1.5, 1.8 and 2.1 mmol/L, respectively. The cell viability was measured by MTT method after incubation at 12, 24, 48 and 72 h. Hoechst stain was used to observe the morphological changes of apoptosis. PC12 cells cultured after 48 h with different concentrations of ketamine were selected to detect apoptotic rate using flow cytometry and detect the expression of bax and bcl-2 proteins using Western blotting. Results For different concentrations of ketamine, vitality of PC12 cells significantly decreased with increase of the incubation time. Apoptosis was obviously observed using Hoechst staining. Flow cytometry showed that apoptosis rates significantly increased with increasing ketamine concentrations. Conclusion Ketamine can inhibit the proliferation of PC12 cell by inducing apoptosis of the PC12 cell in a concentrations-dependent manner. The underlying mechanism may be related to promoting the expression of bax and inhibiting the expression of bcl-2 in the cells.

Key words: forensic pathology, ketamine, PC12 cells, cell proliferation, apoptosis, rats

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